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Membrane Expression Enhances Folding, Multimeric Structure Formation, and Immunogenicity of Viral Capsid Proteins
Open accessPublished version of a preprint
Abstract
Viral capsid proteins are widely explored for subunit vaccine development but are often hampered by their complexity of production and low immunogenicity. Here, we report a simple approach to overcoming these challenges by combining mRNA vaccine technology with protein engineering. Using African swine fever virus (ASFV) capsid proteins P72 and penton as models, we engineered them into membrane-bound and secreted forms and compared their immunogenicity to that of the native intracellular form in mice and pigs through mRNA vaccination. The membrane-bound and secreted P72 and penton folded into their native multimeric structure independent of the viral chaperone, therefore preserving their conformational epitopes. The membrane-bound P72 and penton also elicited significantly stronger antibody and T cell responses than their secreted or intracellular counterparts. Our study provides a simple approach to enhancing folding, multimeric structure formation, and immunogenicity of viral capsid proteins for ASFV subunit vaccine development and immunogenicity of intracellular proteins in general.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Single-Shot Ad26 Vaccine Protects Against SARS-CoV-2 in Rhesus Macaques
Mercado, N. B., Zahn, R., Wegmann, F., Loos, C., Chandrashekar, A., Yu, J., Liu, J., Peter, L., McMahan, K., Tostanoski, L. H., et al. (2020) · Nature · doi:10.1038/s41586-020-2607-z · Cited by 789
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Metadata
- Authors
- Cui, J., Yuan, F., Qin, J., Jeon, J. H., Yun, D. S., Wang, T., Xu, R., Cao, H., Tungate, A. A., Netherton, C. L., & Chen, J.
- Affiliations
- Koch Institute for Integrative Cancer Research At MIT (US)
- Advanced RNA Vaccine Technologies, Inc., North Bethesda, Rockville, Maryland 20852, United States
- InnovHope, Inc.
- The Pirbright Institute (GB)
- Corresponding author
- Chen, Jianzhu
- ORCID
- Venue
- ACS Infectious Diseases
- Publisher
- American Chemical Society (ACS)
- Published
- 2025-07-09
- Volume
- 11
- Issue
- 8
- Pages
- 2104-2115
- ISSN
- 2373-8227 (ISSN-L)
- PMC
- PMC12341548
- OpenAlex
- W4412124559
- Cited by
- 1
- References
- 2
- Citations by year
- 2025: 1
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- ACS Infectious Diseases (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (submittedVersion)
- Funders
- Division of Cancer Prevention, National Cancer Institute (P30-CA14051)
- National Institute of Food and Agriculture (2024-67012-42721)
- New Hope Group Singapore
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Disease Management and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Immunology and Microbiology › Virology › Poxvirus research and outbreaks
- Keywords
- capsid protein, membrane proteins, secreted proteins, protein folding, multimeric structure, immunogenicity, mRNA vaccination, subunit vaccine, African swine fever virus, protein engineering, antibody response, T cell response, pigs
- MeSH terms
- Immunogenicity, Vaccine, African Swine Fever Virus, Animals, Antibodies, Viral, Female, Mice, Inbred BALB C, Swine, Viral Vaccines, Protein Engineering, Protein Folding, Vaccines, Subunit, Capsid Proteins, Mice
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