The DOI matches its Crossref record.
An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug–Protein Binding
Open accessAbstract
An inexpensive, in-house made microdialysis device is described that is suitable for measuring the binding of small molecules including drug candidates to serum proteins or other macromolecules. The device is based on the standard equilibrium dialysis method to measure the fraction of low molecular weight compound bound to proteins. It is constructed from a standard polypropylene 96-well plate, dialysis tubing, and low viscosity epoxy resin. The device can be readily prepared for a small fraction of the cost of a commercial, multichamber microdialysis device. Drug-protein binding results are provided, which validates the device.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Deployment of an ED technique adds extra costs to clinical laboratory practice.
“In addition to these technical issues, deployment of an ED technique adds extra costs to the clinical laboratory practice [227].”
From Role of sex hormone-binding globulin in the free hormone hypothesis and the relevance of free testosterone in androgen physiology (2022) · cites this paper as [227]
The discussion accompanying Table 1 reports that commercial equilibrium-dialysis devices cost approximately $5–10 per chamber and describes them as high-priced compared with the in-house device. This supports a cost concern, but the paper discusses drug-binding research devices, not the added costs of deploying equilibrium dialysis in clinical laboratory practice. (LLM, conf=0.83)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 15
- 10.1002/1520-6017(200105)90:5<580::AID-JPS1014>3.0.CO;2-4 · DOI
- Wang W., 2016, ADME and Translational Pharmacokinetics/Pharmacodynamics of Therapeutic Proteins: Applications in Drug Discovery and Development, 1
- Cohen L. H., 2004, Methods in Pharmacology and Toxicology Optimization in Drug Discovery: In Vitro Methods, 111, 10.1385/1-59259-800-5:111 · DOI
- 10.1016/j.jala.2010.06.002 · DOI
- 10.1002/jps.21317 · DOI
- 10.1002/jps.10332 · DOI
- 10.2165/00003088-199019040-00002 · DOI
- Kapoor V. K., 1994, Analytical Profiles of Drug Substances and Excipients, 23, 571
- 10.1016/j.pharmthera.2016.04.010 · DOI
- 10.1016/0022-4731(82)90038-3 · DOI · Paper page
- Skibińska L., 1990, Polym. J. Pharmacol. Pharm., 42, 151, 10.1111/j.2042-7158.1990.tb14524.x · DOI
- 10.1124/dmd.110.036988 · DOI
- 10.1002/jps.2600760204 · DOI
- Kumar G. N., 1993, Res. Communc. Chem. Pathol. Pharmacol., 80, 337
- Khurana, I. Medical Physiology for Undergraduate Students , 2nd ed. Elsevier: India, 2011, 861.
Cited by
Sorted by citations
Narinx, N., David, K., Walravens, J., Vermeersch, P., Claessens, F., Fiers, T., Lapauw, B., Antonio, L., & Vanderschueren, D. (2022) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-022-04562-1 · Cited by 96
Metadata
- Authors
- Herbst, Z. M., Shibata, S., Fan, E., & Gelb, M. H.
- Affiliations
- University of Washington (US)
- Corresponding author
- Gelb, Michael H.
- Venue
- ACS Medicinal Chemistry Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-09-28
- Volume
- 9
- Issue
- 3
- Pages
- 279-282
- ISSN
- 1948-5875 (ISSN-L)
- PMC
- PMC5846030
- OpenAlex
- W2759902082
- Cited by
- 5
- References
- 0
- Citations by year
- 2024: 1 · 2022: 2 · 2020: 1 · 2019: 1
- FWCI
- 0.22
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- ACS Medicinal Chemistry Letters (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI070218, AI097177)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Keywords
- drug-protein binding, equilibrium dialysis, low-cost laboratory equipment, serum proteins, drug candidates, epoxy resin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
