The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a
Abstract
Highly infectious illness caused by pathogens is endemic especially in developing nations where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care (POC) serological assays with minimal sample manipulation and low cost are desired in clinical practice. In this study, we report an automated POC system for Ebola RNA detection with RNA-guided RNA endonuclease Cas13a, utilizing its collateral RNA degradation after its activation. After automated microfluidic mixing and hybridization, nonspecific cleavage products of Cas13a are immediately measured by a custom integrated fluorometer which is small in size and convenient for in-field diagnosis. Within 5 min, a detection limit of 20 pfu/mL (5.45 × 10 7 copies/mL) of purified Ebola RNA is achieved. This isothermal and fully solution-based diagnostic method is rapid, amplification-free, simple, and sensitive, thus establishing a key technology toward a useful POC diagnostic platform.
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Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
Rapid Molecular Detection of Extrapulmonary Tuberculosis by the Automated GeneXpert MTB/RIF System
Hillemann, D., Rüsch-Gerdes, S., Boehme, C., & Richter, E. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.02268-10 · Cited by 413
Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Point-of-care diagnostics for infectious diseases: From methods to devices
Wang, C., Liu, M., Wang, Z., Li, S., Deng, Y., & He, N. (2021) · Nano Today · doi:10.1016/j.nantod.2021.101092 · Cited by 622
Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Li, H., Xie, Y., Chen, F., Bai, H., Xiu, L., Zhou, X., Guo, X., Hu, Q., & Yin, K. (2023) · Chemical Society Reviews · doi:10.1039/d2cs00594h · Cited by 346
Tian, T., Shu, B., Jiang, Y., Ye, M., Liu, L., Guo, Z., Han, Z., Wang, Z., & Zhou, X. (2021) · ACS Nano · doi:10.1021/acsnano.0c08165 · Cited by 328
Feng, W., Newbigging, A. M., Tao, J., Cao, Y., Peng, H., Le, C., Wu, J., Pang, B., Li, J., Tyrrell, D. L., et al. (2021) · Chemical Science · doi:10.1039/d0sc06973f · Cited by 312
Metadata
- Authors
- Qin, P., Park, M., Alfson, K. J., Tamhankar, M., Carrion, R., Patterson, J. L., Griffiths, A., He, Q., Yildiz, A., Mathies, R., & Du, K.
- Venue
- ACS Sensors
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-03-12
- Volume
- 4
- Issue
- 4
- Pages
- 1048-1054
- ISSN
- 2379-3694 (ISSN-L)
- OpenAlex
- W2920964100
- Cited by
- 311
- References
- 3
- FWCI
- 12.60
- Type
- journal article
- Language
- en
- Open access
- closed
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- Qin, P., Park, M., Alfson, K. J., Tamhankar, M., Carrion, R., Patterson, J. L., Griffiths, A., He, Q., Yildiz, A., Mathies, R., & Du, K. (2019). Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a. ACS Sensors. https://doi.org/10.1021/acssensors.9b00239
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