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Alternating-Laser Excitation of Single Molecules
Abstract
Single-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interactions, and dynamics. This is accomplished using ratios that report on the distance between and the stoichiometry of fluorophores attached to the molecules of interest. The principle of alternation is compatible with several time scales, allowing monitoring of fast dynamics or simultaneous monitoring of a large number of individual molecules.
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Cited by
Extreme disorder in an ultrahigh-affinity protein complex
Borgia, A., Borgia, M. B., Bugge, K., Kissling, V. M., Heidarsson, P. O., Fernandes, C. B., Sottini, A., Soranno, A., Buholzer, K. J., Nettels, D., et al. (2018) · Nature · doi:10.1038/nature25762 · Cited by 653
Shot-Noise Limited Single-Molecule FRET Histograms: Comparison between Theory and Experiments
Nir, E., Michalet, X., Hamadani, K. M., Laurence, T. A., Neuhauser, D., Kovchegov, Y., & Weiss, S. (2006) · The Journal of Physical Chemistry B · doi:10.1021/jp063483n · Cited by 314
Self-citationAccurate Single-Molecule FRET Studies Using Multiparameter Fluorescence Detection
Sisamakis, E., Valeri, A., Kalinin, S., Rothwell, P. J., & Seidel, C. A. M. (2010) · Methods in Enzymology · doi:10.1016/s0076-6879(10)75018-7 · Cited by 268
Labeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics
Tian, H., Fürstenberg, A., & Huber, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00084 · Cited by 114
Dynamic action of the Sec machinery during initiation, protein translocation and termination.
Fessl, T., Watkins, D., Oatley, P., Allen, W. J., Corey, R. A., Horne, J., Baldwin, S. A., Radford, S. E., Collinson, I., & Tuma, R. (2018) · eLife · doi:10.7554/elife.35112 · Cited by 56
Metadata
- Authors
- Kapanidis, A. N., Laurence, T. A., Lee, N. K., Margeat, E., Kong, X., & Weiss, S.
- Venue
- Accounts of Chemical Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-04-02
- Volume
- 38
- Issue
- 7
- Pages
- 523-533
- ISSN
- 0001-4842 (ISSN-L)
- 1520-4898
- OpenAlex
- W2119562650
- Cited by
- 358
- References
- 0
- FWCI
- 8.64
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
