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Selective Screening Methods for the Isolation of High Yielding Cellulase Mutants of Trichoderma reesei

MONTENECOURT, BLAND S., EVELEIGH, DOUGLAS E.·1979·Advances in Chemistry

Abstract

Screening methods for the selection of high-yielding cellulase mutants of Trichoderma reesei have been devised that specifically detect hyperproducing, catabolite repression-resistant, and end-product inhibition-resistant strains. Individual selection techniques employing specific substrates and end-product inhibitors allow for visual screening of a single enzyme in the cellulase complex: endo-β 1-4 glucanase and β-glucosidase (cellobiase) as well as the synergistic action of all the enzymes of the cellulase complex. By use of these methods, mutant strains have been isolated that hyperproduce cellulase and are resistant to catabolite repression by glucose or glycerol.

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Metadata

Authors
MONTENECOURT, B. S., & EVELEIGH, D. E.
Venue
Advances in Chemistry
Published
1979-06-01
Pages
289-301
ISSN
0065-2393 (ISSN-L)
OpenAlex
W2494850533
Cited by
248
References
0
FWCI
10.67
Type
book chapter
Language
en
Open access
closed

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CoChat. (2026). Citation check: Selective Screening Methods for the Isolation of High Yielding Cellulase Mutants of Trichoderma reesei. https://app.cochat.ai/papers/10.1021/ba-1979-0181.ch014
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MONTENECOURT, B. S., & EVELEIGH, D. E. (1979). Selective Screening Methods for the Isolation of High Yielding Cellulase Mutants of Trichoderma reesei. Advances in Chemistry. https://doi.org/10.1021/ba-1979-0181.ch014

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