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Enzymatic Modification of Self-Assembled Peptide Structures with Tissue Transglutaminase
Abstract
A de novo peptide that self-assembles into fibrillar structures and serves as a substrate for the cross-linking enzyme tissue transglutaminase was developed (Ac-QQKFQFQFEQQ-Am). Congo red staining, circular dichroism, and FTIR spectroscopy showed that this 11-amino acid peptide produced predominantly beta-sheet structures. TEM with negative staining and quick-freeze deep etch (QFDE) TEM showed that the peptide structures were composed of a highly entangled fibrillar network. These beta-sheet fibrillar nanostructures were then covalently coupled to pendant amine-containing biomolecules via tissue transglutaminase. MALDI-TOF mass spectrometry and HPLC were utilized to monitor the extent of the transglutaminase modification of the peptide, showing that as many as five glutamines in the peptide were reactive via transglutaminase for covalent conjugation. This strategy, based on the post-assembly modification of a self-assembling peptide, has potential applications for tailoring supramolecular structures for drug delivery, tissue engineering, or other biomedical applications.
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Cited by
Biomaterials: Where We Have Been and Where We Are Going
Ratner, B. D., & Bryant, S. J. (2004) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.6.040803.140027 · Cited by 1,507
Yi, H., Wu, L.-Q., Bentley, W. E., Ghodssi, R., Rubloff, G. W., Culver, J. N., & Payne, G. F. (2005) · Biomacromolecules · doi:10.1021/bm050410l · Cited by 759
Smart polymeric gels: Redefining the limits of biomedical devices
Chaterji, S., Kwon, I. K., & Park, K. (2007) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2007.05.018 · Cited by 615
McHale, M. K., Setton, L. A., & Chilkoti, A. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.1768 · Cited by 295
Metadata
- Authors
- Collier, J. H., & Messersmith, P. B.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-06-24
- Volume
- 14
- Issue
- 4
- Pages
- 748-755
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W2161417492
- Cited by
- 171
- References
- 0
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
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- Collier, J. H., & Messersmith, P. B. (2003). Enzymatic Modification of Self-Assembled Peptide Structures with Tissue Transglutaminase. Bioconjugate Chemistry. https://doi.org/10.1021/bc034017t
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