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Site-Specific Polymer−Streptavidin Bioconjugate for pH-Controlled Binding and Triggered Release of Biotin
Abstract
Low molecular weight copolymers of acrylic acid (AAc) and N-isopropylacrylamide (NIPAAm) have been synthesized with reactive OH groups at one end, using a chain transfer polymerization technique. The copolymer displays both pH and temperature sensitivity over a wide and useful range of pHs and temperatures, which permits both pH and temperature control of polymer conformation. This copolymer has been conjugated to a specific cysteine thiol site inserted by genetic engineering near the recognition site of streptavidin (SAv). In this paper, we demonstrate that this bioconjugate can provide pH control of biotin binding to and triggered release from the mutant SAv. These actions are relevant to affinity separations, biosensors, diagnostics, enzyme processes, and targeted delivery of drugs or chemical agents, labels, and other signals.
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Citations
Cited by
Stimuli-reponsive polymers and their bioconjugates
GIL, E., & HUDSON, S. (2004) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2004.08.003 · Cited by 2,385
Bio‐Inspired, Smart, Multiscale Interfacial Materials
Xia, F., & Jiang, L. (2008) · Advanced Materials · doi:10.1002/adma.200800836 · Cited by 963
Stimuli-responsive polymers: Biomedical applications and challenges for clinical translation
Hoffman, A. S. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.11.004 · Cited by 759
Self-citationWell-Defined Protein−Polymer Conjugates via in Situ RAFT Polymerization
Boyer, C., Bulmus, V., Liu, J., Davis, T. P., Stenzel, M. H., & Barner-Kowollik, C. (2007) · Journal of the American Chemical Society · doi:10.1021/ja070956a · Cited by 411
Self-citationStability of responsive polymer–protein bioconjugates
Shakya, A. K., Sami, H., Srivastava, A., & Kumar, A. (2010) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2010.01.003 · Cited by 110
Metadata
- Authors
- Bulmus, V., Ding, Z., Long, C. J., Stayton, P. S., & Hoffman, A. S.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-12-03
- Volume
- 11
- Issue
- 1
- Pages
- 78-83
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W2054184600
- Cited by
- 203
- References
- 0
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bulmus, V., Ding, Z., Long, C. J., Stayton, P. S., & Hoffman, A. S. (2000). Site-Specific Polymer−Streptavidin Bioconjugate for pH-Controlled Binding and Triggered Release of Biotin. Bioconjugate Chemistry. https://doi.org/10.1021/bc9901043
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