The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Oxidative Stress Induces Intracellular Accumulation of Amyloid β-Protein (Aβ) in Human Neuroblastoma Cells
Abstract
Several lines of evidence suggest that enhanced oxidative stress is involved in the pathogenesis and/or progression of Alzheimer's disease (AD). Amyloid beta-protein (Abeta) that composes senile plaques, a major neuropathological hallmark of AD, is considered to have a causal role in AD. Thus, we have studied the effect of oxidative stress on Abeta metabolism within the cell. Here, we report that oxidative stress induced by H(2)O(2) (100-250 microM) caused an increase in the levels of intracellular Abeta in human neuroblastoma SH-SY5Y cells. Treatment with 200 microM H(2)O(2) caused significant decreases in the protein levels of full-length beta-amyloid precursor protein (APP) and its COOH-terminal fragment that is generated by beta-cleavage, while the gene expression of APP was not altered under these conditions. A pulse-chase experiment further showed a decrease in the half-life of this amyloidogenic COOH-terminal fragment but not in that of nonamyloidogenic counterpart in the H(2)O(2)-treated cells. These results suggest that oxidative stress promotes intracellular accumulation of Abeta through enhancing the amyloidogenic pathway.
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“Evidence also suggests that not only does Aβ increase cellular RS but that these RS may in turn increase production of Aβ in a destructive autocatalytic cycle (Misonou et al., 2000; Tamagno et al., 2002, 2005; Lin and Beal, 2006; Quiroz-Baez et al., 2009).”
From The mitochondria-targeted antioxidant MitoQ prevents loss of spatial memory retention and early neuropathology in a transgenic mouse model of Alzheimer’s disease (2011) · cites this paper as Misonou et al., 2000
In the citing paper
“Aβ appears to enhance production of cellular RS, and these RS in turn may to contribute to Aβ toxicity.”
From The mitochondria-targeted antioxidant MitoQ prevents loss of spatial memory retention and early neuropathology in a transgenic mouse model of Alzheimer’s disease (2011) · cites this paper as Misonou et al., 2000
In the citing paper
“On the other hand, many experimental data suggest that oxidative stress and mitochondrial dysfunction induce an increase in the amyloidogenic cleavage of the APP and an aberrant Aβ production (Misonou et al., 2000; Perez Ortiz and Swerdlow, 2019).”
From Exploring the Therapeutic Potential of Phytochemicals in Alzheimer’s Disease: Focus on Polyphenols and Monoterpenes (2022) · cites this paper as Misonou et al., 2000
Citations
Cites
Mosmann, T. (1983) · Journal of Immunological Methods · doi:10.1016/0022-1759(83)90303-4 · Cited by 56,104
Iwatsubo, T., Odaka, A., Suzuki, N., Mizusawa, H., Nukina, N., & Ihara, Y. (1994) · Neuron · doi:10.1016/0896-6273(94)90458-8 · Cited by 1,774
Self-citationSayre, L., Zelasko, D. A., Harris, P. L. R., Perry, G., Salomon, R. G., & Smith, M. A. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.68052092.x · Cited by 1,032
Lyras, L., Cairns, N. J., Jenner, A., Jenner, P., & Halliwell, B. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.68052061.x · Cited by 531
The promoter of Alzheimer's disease amyloid A4 precursor gene.
Salbaum, J. M., Weidemann, A., Lemaire, H.-G., Masters, C. L., & Beyreuther, K. T. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb03136.x · Cited by 311
Skovronsky, D. M., Doms, R. W., & Lee, V. M.-Y. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.141.4.1031 · Cited by 290
Cerebrospinal fluid F2‐isoprostane levels are increased in Alzheimer's disease
Montine, T., Markesbery, W., Morrow, J., & Roberts, L. (1998) · Annals of Neurology · doi:10.1002/ana.410440322 · Cited by 254
Frederikse, P. H., Garland, D. L., Zigler, J. S., & Piatigorsky, J. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.17.10169 · Cited by 190
F4 ‐ Isoprostanes as Specific Marker of Docosahexaenoic Acid Peroxidation in Alzheimer's Disease
Nourooz‐Zadeh, J., Liu, E. H. C., Yhlen, B., Änggåard, E. E., & Halliwell, B. (1999) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1999.0720734.x · Cited by 187
Avdulov, N. A., Chochina, S. V., Igbavboa, U., Warden, C. S., Vassiliev, A. V., & Wood, W. G. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.69041746.x · Cited by 180
Flagged first, then by citations
Research works printed but not linked: 34
- Ames B. N., 1993, Proc. Natl. Acad. Sci. U.S.A. 90, 7915−7922
- Markesbery W. R., 1999, Brain Pathol. 9, 133−146
- Mecocci P., 1994, Ann. Neurol. 36, 747−751
- Nonomura A., 1999, J. Neurosci., 19
- Smith M. A., 1996, Nature 382, 120−121
- Smith M. A., 1997, J. Neurosci., 17
- Smith M. A., 1994, Am. J. Pathol., 145, 47
- Schipper H. M., 1995, Ann. Neurol. 37, 758−768
- Smith M. A., 1997, Proc. Natl. Acad. Sci. U.S.A. 94, 9866−9868
- Yan S. D., 1995, 1, 693−699
- Zhang L., 1997, Biochem. Biophys. Res. Commun. 235, 845−848
- Lee S.-J., 1998, 4, 730−734
- Morishima-Kawashima M., 1998, Biochemistry 37, 15247−15253
- Koo E. H., 1994, J. Biol. Chem., 269
- Koopman G., 1994, Blood 84, 1415−1420
- Ida N., 1996, J. Biol. Chem., 271
- Rao P. S., 1988, Biochem. Biophys. Res. Commun., 150, 44
- Selkoe D. J., 1999, A23−31.
- Vassar R., 1999, Science 286, 735−741
- Karin M., 1992, Trends Biochem. Sci. 17, 418−422
- Behl C., 1994, Cell 77, 817−827
- Estus S., 1992, Science 255, 726−728
- Ida N., 1996, FEBS Lett., 394
- LeBlanc A., 1999, J. Biol. Chem., 274
- Tanzi R. E., 1999, Nat. Neurosci. 2, 585−586
- Simons K., 1997, Nature 387, 569−572
- Choo-Smith L.-P., 1997, J. Biol. Chem., 272
- Brown D. A., 1992, Cell 68, 533−544
- Funato H., 1998, Am. J. Pathol., 152
- Lue L. F., 1999, Am. J. Pathol., 155
- Yan S. D., 1999, J. Biol. Chem., 274
- Bame K. J., 1997, J. Biol. Chem., 272
- Goedert M., 1996, Nature 383, 550−553
- Abbreviations AD, Alzheimer's disease
Cited by
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Items 301–303 of 303
Palabıyık, Ş. S., Akpınar, E., & Halıcı, Z. (2026) · Research Square · doi:10.21203/rs.3.rs-10918362/v1
The WUTC threshold test: a new perspective in olfactory assessment
Tewalt, W. A. (2013) · UTC Scholar (University of Tennessee at Chattanooga)
Lysosomal Involvement in the Pathogenesis of Alzheimer's Disease
Zheng, L. P. (2012) · DiVA (Linkoping University)
Metadata
- Authors
- Misonou, H., Morishima-Kawashima, M., & Ihara, Y.
- Affiliations
- Japan Science and Technology Agency (JP)
- Department of Neuropathology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Kawaguchi, Saitama 332-0012, Japan
- Corresponding author
- Ihara, Yasuo
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-05-19
- Volume
- 39
- Issue
- 23
- Pages
- 6951-6959
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2048360633
- Cited by
- 304
- References
- 19
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 11 · 2023: 12 · 2022: 6 · 2021: 9 · 2020: 9 · 2019: 8 · 2018: 19 · 2017: 14 · 2016: 12 · 2015: 10 · 2014: 13 · 2013: 8 · 2012: 15
- FWCI
- 6.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Keywords
- oxidative stress, amyloid-beta, Alzheimer's disease, hydrogen peroxide, amyloid precursor protein, APP cleavage, amyloidogenic pathway, intracellular accumulation, SH-SY5Y cells, protein half-life, amyloid plaques
- MeSH terms
- Alzheimer Disease, Animals, Antioxidants, Cell Survival, Chromans, Dimethyl Sulfoxide, Cricetinae, Humans, Hydrogen Peroxide, Neuroblastoma, Peptide Fragments, Phosphatidylserines, RNA, Messenger, Tetrazolium Salts, Thiazoles, Transfection, Tumor Cells, Cultured, Amyloid beta-Peptides, CHO Cells, Amyloid beta-Protein Precursor, Apoptosis, Oxidative Stress
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