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The 43-kilodalton N-terminal fragment of the DNA gyrase B protein hydrolyzes ATP and binds coumarin drugs
Abstract
We have cloned and overexpressed a gene encoding a 43-kDa protein corresponding to the N-terminal fragment of the DNA gyrase B subunit. We show that this protein hydrolyzes ATP and binds coumarin drugs. The hydrolysis of ATP shows distinctly non-Michaelis-Menten kinetics and is consistent with a scheme in which the active form of the protein is a dimer, a conclusion supported by molecular weight studies. The coumarin drugs bind very tightly to the 43-kDa fragment, with novobiocin binding to the protein monomer and coumermycin A1 apparently inducing the formation of a dimer. The implications of these results with respect to the mechanism of supercoiling by DNA gyrase and the inhibition of gyrase by coumarin drugs are discussed.
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Citations
Cited by
ATP binding and hydrolysis are essential to the function of the Hsp90 molecular chaperone in vivo
Panaretou, B., Prodromou, C., Roe, S. M., O'Brien, R., Ladbury, J. E., Piper, P. W., & Pearl, L. H. (1998) · The EMBO Journal · doi:10.1093/emboj/17.16.4829 · Cited by 735
DNA topoisomerases: harnessing and constraining energy to govern chromosome topology
Schoeffler, A. J., & Berger, J. M. (2008) · Quarterly Reviews of Biophysics · doi:10.1017/s003358350800468x · Cited by 490
Dissection of the ATP-induced conformational cycle of the molecular chaperone Hsp90
Hessling, M., Richter, K., & Buchner, J. (2009) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1565 · Cited by 349
Lewis, R. J., Singh, O. M., Smith, C. V., Skarzynski, T., Maxwell, A., Wonacott, A. J., & Wigley, D. B. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00483.x · Cited by 335
Self-citationChemically Controlled Protein Assembly: Techniques and Applications
Fegan, A., White, B., Carlson, J. C. T., & Wagner, C. R. (2010) · Chemical Reviews · doi:10.1021/cr8002888 · Cited by 294
Metadata
- Authors
- Ali, J. A., Jackson, A. P., Howells, A. J., & Maxwell, A.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1993-03-16
- Volume
- 32
- Issue
- 10
- Pages
- 2717-2724
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2068435780
- Cited by
- 303
- References
- 0
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ali, J. A., Jackson, A. P., Howells, A. J., & Maxwell, A. (1993). The 43-kilodalton N-terminal fragment of the DNA gyrase B protein hydrolyzes ATP and binds coumarin drugs. Biochemistry. https://doi.org/10.1021/bi00061a033
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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