CoChat
Sign Up
Cited by 459

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

The Quantitatively Important Relationship between Homocysteine Metabolism and Glutathione Synthesis by the Transsulfuration Pathway and Its Regulation by Redox Changes

Mosharov, Eugene, Cranford, Matthew R., Banerjee, Ruma·2000·Biochemistry
DOI 10.1021/bi001088w
Open this paper in CoChat — free account

Abstract

Homocysteine is a key junction metabolite in methionine metabolism. It suffers two major metabolic fates: transmethylation catalyzed by methionine synthase or betaine homocysteine methyl transferase and transsulfuration catalyzed by cystathionine beta-synthase leading to cystathionine. The latter is subsequently converted to cysteine, a precursor of glutathione. Studies with purified mammalian methionine synthase and cystathionine beta-synthase have revealed the oxidative sensitivity of both junction enzymes, suggesting the hypothesis that redox regulation of this pathway may be physiologically significant. This hypothesis has been tested in a human hepatoma cell line in culture in which the flux of homocysteine through transsulfuration under normoxic and oxidative conditions has been examined. Addition of 100 microM H(2)O(2) or tertiary butyl hydroperoxide increased cystathionine production 1.6- and 2.1-fold from 82 +/- 7 micromol h(-)(1) (L of cells)(-)(1) to 136 +/- 15 and 172 +/- 23 micromol h(-)(1) (L of cells)(-)(1), respectively. The increase in homocysteine flux through the transsulfuration pathway exhibited a linear dose dependence on the concentrations of both oxidants (50-200 microM H(2)O(2) and 10-200 microM tertiary butyl hydroperoxide). Furthermore, our results reveal that approximately half of the intracellular glutathione pool in human liver cells is derived from homocysteine via the transsulfuration pathway. The redox sensitivity of the transsulfuration pathw…

Overview

Integrity signals

Registry

Crossref · 10.1021/bi001088w

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 26

  1. Mills J. L., 1995, Lancet 345, 149−151
  2. Steegers-Theunissen R. P., 1994, Metabolism 43, 1475−1480
  3. Clarke R., 1998, Arch. Neruol. 55, 1449−1455
  4. Finkelstein J. D., 1990, J. Nutr. Biochem., 1, 10.1016/0955-2863(90)90070-2 · DOI
  5. Pajares M. A., 1992, J. Biol. Chem., 267, 10.1016/S0021-9258(19)37084-X · DOI
  6. Ruiz F., 1998, Hepatology 28, 1051−1057
  7. Chen Z., 1995, J. Biol. Chem., 270
  8. Taoka S., 1998, J. Biol. Chem., 273, 10.1074/jbc.273.39.25179 · DOI
  9. Reed D., 1980, Anal. Biochem., 106, 62, 10.1016/0003-2697(80)90118-9 · DOI
  10. Abeles R. H., 1973, J. Am. Chem. Soc., 95, 10.1021/ja00799a053 · DOI
  11. Rao A. M., 1990, J. Nutr., 120
  12. Nose K., 1991, Eur. J. Biochem., 201, 106, 10.1111/j.1432-1033.1991.tb16261.x · DOI
  13. Meyer M., 1993, EMBO J., 12, 10.1002/j.1460-2075.1993.tb05850.x · DOI
  14. Jakubowski H., 1997, J. Biol. Chem., 272, 10.1016/S0021-9258(19)67504-6 · DOI
  15. Taoka S., 1999, Biochemistry 38, 13155−13161
  16. Kery V., 1998, Arch. Biochem. Biophys. 355, 222−232
  17. Skovby F., 1984, J. Biol. Chem., 259
  18. Iwata-Ichikawa E., 1999, J. Neurochem., 72, 10.1046/j.1471-4159.1999.0722334.x · DOI
  19. Beatty P. W., 1980, Arch. Biochem. Biophys., 204, 87, 10.1016/0003-9861(80)90009-0 · DOI
  20. Mudd S. H., 1995, in The Metabolic and molecular basis of inherited diseases
  21. Irreverre F., 1967, Biochem. Med., 1, 187, 10.1016/0006-2944(67)90007-5 · DOI
  22. Raghavachari N., 1999, Exp. Eye Res. 68, 715−724
  23. Finkelstein J. D., 1984, J. Biol. Chem., 259, 10.1016/S0021-9258(17)42728-1 · DOI
  24. Lee C.-K., 1999, Science 285, 1390−1393
  25. Abbreviations, tetrahydrofolate
  26. The, The means are significantly different at the

Cited by

Sorted by citations

Metadata

Authors
Mosharov, E., Cranford, M. R., & Banerjee, R.
Affiliations
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664
Venue
Biochemistry
Publisher
American Chemical Society (ACS)
Published
2000-09-23
Volume
39
Issue
42
Pages
13005-13011
ISSN
  • 0006-2960
  • 1520-4995
DOI
10.1021/bi001088w
Cited by
459
References
1
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review