The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Analogs of Reaction Intermediates Identify a Unique Substrate Binding Site in Candida rugosa Lipase
Abstract
The structures of Candida rugosa lipase-inhibitor complexes demonstrate that the scissile fatty acyl chain is bound in a narrow, hydrophobic tunnel which is unique among lipases studied to date. Modeling of triglyceride binding suggests that the bound lipid must adopt a "tuning fork" conformation. The complexes, analogs of tetrahedral intermediates of the acylation and deacylation steps of the reaction pathway, localize the components of the oxyanion hole and define the stereochemistry of ester hydrolysis. Comparison with other lipases suggests that the positioning of the scissile fatty acyl chain and ester bond and the stereochemistry of hydrolysis are the same in all lipases which share the alpha/beta-hydrolase fold.
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Nicholls, A., Sharp, K. A., & Honig, B. (1991) · Proteins: Structure, Function, and Bioinformatics · doi:10.1002/prot.340110407 · Cited by 5,240
Sussman, J. L., Harel, M., Frolow, F., Oefner, C., Goldman, A., Toker, L., & Silman, I. (1991) · Science · doi:10.1126/science.1678899 · Cited by 2,707
Analytical molecular surface calculation
Connolly, M. L. (1983) · Journal of Applied Crystallography · doi:10.1107/s0021889883010985 · Cited by 2,508
Ollis, D. L., Cheah, E., Cygler, M., Dijkstra, B., Frolow, F., Franken, S. M., Harel, M., Remington, S. J., Silman, I., Schrag, J., et al. (1992) · "Protein Engineering, Design and Selection" · doi:10.1093/protein/5.3.197 · Cited by 2,120
Self-citationA graphics model building and refinement system for macromolecules
Jones, T. A. (1978) · Journal of Applied Crystallography · doi:10.1107/s0021889878013308 · Cited by 1,839
Structure of human pancreatic lipase
Winkler, F. K., D'Arcy, A., & Hunziker, W. (1990) · Nature · doi:10.1038/343771a0 · Cited by 1,224
A serine protease triad forms the catalytic centre of a triacylglycerol lipase
Brady, L., Brzozowski, A. M., Derewenda, Z. S., Dodson, E., Dodson, G., Tolley, S., Turkenburg, J. P., Christiansen, L., Huge-Jensen, B., Norskov, L., et al. (1990) · Nature · doi:10.1038/343767a0 · Cited by 1,218
Brzozowski, A. M., Derewenda, U., Derewenda, Z. S., Dodson, G. G., Lawson, D. M., Turkenburg, J. P., Bjorkling, F., Huge-Jensen, B., Patkar, S. A., & Thim, L. (1991) · Nature · doi:10.1038/351491a0 · Cited by 1,152
The α-helix dipole and the properties of proteins
Hol, W. G. J., van Duijnen, P. T., & Berendsen, H. J. C. (1978) · Nature · doi:10.1038/273443a0 · Cited by 998
van Tilbeurgh, H., Egloff, M.-P., Martinez, C., Rugani, N., Verger, R., & Cambillau, C. (1993) · Nature · doi:10.1038/362814a0 · Cited by 686
Cited by
Jendrossek, D. (1998) · Polymer Degradation and Stability · doi:10.1016/s0141-3910(97)00190-0 · Cited by 95
Briese, B. H., & Jendrossek, D. (1998) · Macromolecular Symposia · doi:10.1002/masy.19981300119 · Cited by 10
Metadata
- Authors
- Grochulski, P., Bouthillier, F., Kazlauskas, R. J., Serreqi, A. N., Schrag, J. D., Ziomek, E., & Cygler, M.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1994-03-29
- Volume
- 33
- Issue
- 12
- Pages
- 3494-3500
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2048069826
- Cited by
- 262
- References
- 20
- FWCI
- 6.01
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Analogs of Reaction Intermediates Identify a Unique Substrate Binding Site in Candida rugosa Lipase. https://app.cochat.ai/papers/10.1021/bi00178a005
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- Grochulski, P., Bouthillier, F., Kazlauskas, R. J., Serreqi, A. N., Schrag, J. D., Ziomek, E., & Cygler, M. (1994). Analogs of Reaction Intermediates Identify a Unique Substrate Binding Site in Candida rugosa Lipase. Biochemistry. https://doi.org/10.1021/bi00178a005
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
