The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F
Abstract
Endo-beta-N-acetylglucosaminidase F (Endo F) and peptide:N-glycosidase F (PNGase F) were purified from cultures of Flavobacterium meningosepticum by ammonium sulfate precipitation followed by gel filtration on TSK HW-55(S). This system separated the two enzymes and provided PNGase F in a high state of purity, but the basis for the resolution appeared to be hydrophobic interaction and not molecular size. Studies using purified Endo F and PNGase F with defined glycopeptides demonstrated that Endo F was somewhat similar to Endo H in that it hydrolyzed many, but not all, high-mannose and hybrid oligosaccharides, as well as complex biantennary oligosaccharides. PNGase F, in contrast, hydrolyzed all classes of asparagine-linked glycans examined, provided both the alpha-amino and carboxyl groups of the asparagine residue were in peptide linkage. Deglycosylation studies with PNGase F revealed that many proteins in their native conformation were susceptible to this enzyme but that prior denaturation in sodium dodecyl sulfate greatly decreased the amount of enzyme required for complete carbohydrate removal.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
Zsebo, K. M., Wypych, J., McNiece, I. K., Lu, H. S., Smith, K. A., Karkare, S. B., Sachdev, R. K., Yuschenkoff, V. N., Birkett, N. C., Williams, L. R., et al. (1990) · Cell · doi:10.1016/0092-8674(90)90300-4 · Cited by 788
YONEKURA, H., YAMAMOTO, Y., SAKURAI, S., PETROVA, R. G., ABEDIN, M. J., LI, H., YASUI, K., TAKEUCHI, M., MAKITA, Z., TAKASAWA, S., et al. (2003) · Biochemical Journal · doi:10.1042/bj20021371 · Cited by 716
An NMDA receptor ER retention signal regulated by phosphorylation and alternative splicing
Scott, D. B., Blanpied, T. A., Swanson, G. T., Zhang, C., & Ehlers, M. D. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-09-03063.2001 · Cited by 418
The Microtubule-associated Protein Tau Is Extensively Modified with O-linked N-acetylglucosamine
Arnold, C. S., Johnson, G. W., Cole, R. N., Dong, D. L.-Y., Lee, M., & Hart, G. W. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.46.28741 · Cited by 361
Weskamp, G., & Reichardt, L. F. (1991) · Neuron · doi:10.1016/0896-6273(91)90067-a · Cited by 294
Metadata
- Authors
- Tarentino, A. L., Gomez, C. M., & Plummer, T. H.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1985-08-01
- Volume
- 24
- Issue
- 17
- Pages
- 4665-4671
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2031119708
- Cited by
- 1,131
- References
- 1
- FWCI
- 20.86
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tarentino, A. L., Gomez, C. M., & Plummer, T. H. (1985). Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F. Biochemistry. https://doi.org/10.1021/bi00338a028
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