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A simple method for the preparation of homogeneous phospholipid vesicles
Abstract
A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, wich is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.
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Citations
Cited by
Phosphatidic Acid-Mediated Mitogenic Activation of mTOR Signaling
Fang, Y., Vilella-Bach, M., Bachmann, R., Flanigan, A., & Chen, J. (2001) · Science · doi:10.1126/science.1066015 · Cited by 1,055
Antonucci, F., Turola, E., Riganti, L., Caleo, M., Gabrielli, M., Perrotta, C., Novellino, L., Clementi, E., Giussani, P., Viani, P., et al. (2012) · The EMBO Journal · doi:10.1038/emboj.2011.489 · Cited by 328
Differential membrane binding of the human immunodeficiency virus type 1 matrix protein
Zhou, W., & Resh, M. D. (1996) · Journal of Virology · doi:10.1128/jvi.70.12.8540-8548.1996 · Cited by 226
Kasianowicz, J., Benz, R., & McLaughlin, S. (1984) · The Journal of Membrane Biology · doi:10.1007/bf01868942 · Cited by 191
Sarafanov, A. G., Ananyeva, N. M., Shima, M., & Saenko, E. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008046200 · Cited by 116
Metadata
- Authors
- Barenholz, Y., Gibbes, D., Litman, B. J., Goll, J., Thompson, T. E., & Carlson, F. D.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1977-06-14
- Volume
- 16
- Issue
- 12
- Pages
- 2806-2810
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2061216302
- Cited by
- 864
- References
- 0
- FWCI
- 12.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A simple method for the preparation of homogeneous phospholipid vesicles. https://app.cochat.ai/papers/10.1021/bi00631a035
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- Barenholz, Y., Gibbes, D., Litman, B. J., Goll, J., Thompson, T. E., & Carlson, F. D. (1977). A simple method for the preparation of homogeneous phospholipid vesicles. Biochemistry. https://doi.org/10.1021/bi00631a035
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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