The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Mechanism of ethidium bromide fluorescence enhancement on binding to nucleic acids
Abstract
The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated. From an examination of the effect of different solvents on the fluorescence lifetime, quenching of fluorescence by proton acceptors, and the substantial lengthening of lifetime observed upon deuteration of the amino protons, regardless of the medium, we conclude that proton transfer from the excited singlet state is the process primarily responsible for the approximately equal to 3.5-fold increase in the lifetime of free ethidium bromide in going from H2O to D2O; the fact that addition of small amounts of water to nonaqueous solvents decreases the fluorescence whereas addition of small amounts of D2O enhances the fluorescence; and the enhancement of the ethidium bromide triplet state yield on binding to DNA. Other proposed mechanisms are shown to be inconsistent with our findings.
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Citations
Cites
Quenching of fluorescence by oxygen. Probe for structural fluctuations in macromolecules
Lakowicz, J. R., & Weber, G. (1973) · Biochemistry · doi:10.1021/bi00745a020 · Cited by 2,790
Relationship between Absorption Intensity and Fluorescence Lifetime of Molecules
Strickler, S. J., & Berg, R. A. (1962) · The Journal of Chemical Physics · doi:10.1063/1.1733166 · Cited by 2,481
A fluorescent complex between ethidium bromide and nucleic acids
Pecq, J. B. L., & Paoletti, C. (1967) · Journal of Molecular Biology · doi:10.1016/0022-2836(67)90353-1 · Cited by 2,393
Complex formation between ethidium bromide and nucleic acids
Waring, M. J. (1965) · Journal of Molecular Biology · doi:10.1016/s0022-2836(65)80096-1 · Cited by 1,512
Physical and chemical properties of singlet molecular oxygen
Kearns, D. R. (1971) · Chemical Reviews · doi:10.1021/cr60272a004 · Cited by 913 · cited 1×
“These results can be interpreted according to the following reaction scheme (Olmsted and Akashah, 1973; Kearns, 1971) (ISo, IS, and 3S refer to ground state, first excited singlet, and triplet state excited sensitizer molecules, respectively; D = DPI, the singlet oxygen acceptor).”
BackgroundSelf-citation[24] Nanosecond fluorescence spectroscopy of macromolecules
Yguerabide, J. (1972) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(72)26026-8 · Cited by 289
Remarkable solvent effects on the lifetime of 1.DELTA.g oxygen
Merkel, P. B., & Kearns, D. R. (1972) · Journal of the American Chemical Society · doi:10.1021/ja00758a071 · Cited by 216
Self-citationExcited-State Proton-Transfer Reactions. A Deuterium Isotope Effect on Fluorescence
Stryer, L. (1966) · Journal of the American Chemical Society · doi:10.1021/ja00976a004 · Cited by 188
Second Triplet State of Anthracene
Kellogg, R. E. (1966) · The Journal of Chemical Physics · doi:10.1063/1.1726480 · Cited by 162
Evolution in vitro: Sequence and phenotype of a mutant RNA resistant to ethidium bromide
Kramer, F. R., Mills, D. R., Cole, P. E., Nishihara, T., & Spiegelman, S. (1974) · Journal of Molecular Biology · doi:10.1016/0022-2836(74)90047-3 · Cited by 139
Cited by
DNA−Protein Cross-Linking via Guanine Oxidation: Dependence upon Protein and Photosensitizer
Kurbanyan, K., Nguyen, K. L., To, P., Rivas, E. V., Lueras, A. M. K., Kosinski, C., Steryo, M., González, A., Mah, D. A., & Stemp, E. D. A. (2003) · Biochemistry · doi:10.1021/bi020713p · Cited by 53
Metadata
- Authors
- Olmsted, J., & Kearns, D. R.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1977-08-09
- Volume
- 16
- Issue
- 16
- Pages
- 3647-3654
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2011682937
- Cited by
- 567
- References
- 15
- FWCI
- 7.71
- Type
- journal article
- Language
- en
- Open access
- closed
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- Olmsted, J., & Kearns, D. R. (1977). Mechanism of ethidium bromide fluorescence enhancement on binding to nucleic acids. Biochemistry. https://doi.org/10.1021/bi00635a022
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
