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3'-Phosphatase activity in T4 polynucleotide kinase
Abstract
The purification of T4 polynucleotide kinase results in the copurification of an activity which will specifically remove the 3'-terminal phosphate from a variety of deoxyribonucleotides and ribonucleotides in the absence of ATP. This phosphatase activity requires magnesium, has a pH optiumum of 6.0, and is more active with deoxyribonucleotides than ribonucleotides. T4 polynucleotide kinase and the 3'-phosphatase activity copurify by gradient elution column chromatography on DEAE-cellulose, phosphocellulose, and hydroxylapatite. The two activities are included in and comigrate on Sephadex G-200. Polyacrylamide gel electrophoresis at PH 9.2 results in conigration of the two activities together with the major protein band. The two activities respond in parallel to heat inactivation at 35 degrees C and ATP, a substrate for the kinase only, protects both activities from heat inactivation. It is therefore suggested that the two activities are functions of the same protein molecule.
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Cited by
The enzymatic preparation of [α-32P]nucleoside triphosphates, cyclic [32P]AMP, and cyclic [32P]GMP
Walseth, T. F., & Johnson, R. A. (1979) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(79)90122-9 · Cited by 573
Mechanism of ribosome recruitment by hepatitis C IRES RNA
KIEFT, J. S., ZHOU, K., JUBIN, R., & DOUDNA, J. A. (2001) · RNA · doi:10.1017/s1355838201001790 · Cited by 369
Jilani, A., Ramotar, D., Slack, C., Ong, C., Yang, X. M., Scherer, S. W., & Lasko, D. D. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.34.24176 · Cited by 293
Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof
Kim, A., Morato, N. M., Saha, P., Eyimegwu, P. N., Niyaz, A. A., Huang, R., Cooks, R. G., Phelan, R. M., & Lewis, J. C. (2025) · Angewandte Chemie International Edition · doi:10.1002/anie.202503538 · Cited by 7
Metadata
- Authors
- Cameron, V., & Uhlenbeck, O. C.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1977-11-15
- Volume
- 16
- Issue
- 23
- Pages
- 5120-5126
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2017139765
- Cited by
- 275
- References
- 0
- FWCI
- 3.35
- Type
- journal article
- Language
- en
- Open access
- closed
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- Cameron, V., & Uhlenbeck, O. C. (1977). 3'-Phosphatase activity in T4 polynucleotide kinase. Biochemistry. https://doi.org/10.1021/bi00642a027
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