The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell Labeling
Open accessAbstract
Fluorescent proteins (FPs) with far-red excitation and emission are desirable for multicolor labeling and live-animal imaging. We describe E2-Crimson, a far-red derivative of the tetrameric FP DsRed-Express2. Unlike other far-red FPs, E2-Crimson is noncytotoxic in bacterial and mammalian cells. E2-Crimson is brighter than other far-red FPs and matures substantially faster than other red and far-red FPs. Approximately 40% of the E2-Crimson fluorescence signal is remarkably photostable. With an excitation maximum at 611 nm, E2-Crimson is the first FP that is efficiently excited with standard far-red lasers. We show that E2-Crimson has unique applications for flow cytometry and stimulated emission depletion (STED) microscopy.
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A clearer vision for in vivo imaging
Weissleder, R. (2001) · Nature Biotechnology · doi:10.1038/86684 · Cited by 3,839
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Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Shcherbakova, D. M., Baloban, M., Emelyanov, A. V., Brenowitz, M., Guo, P., & Verkhusha, V. V. (2016) · Nature Communications · doi:10.1038/ncomms12405 · Cited by 312
Zebrafish embryos and larvae: A new generation of disease models and drug screens
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Maturation-driven transport and AP-1–dependent recycling of a secretory cargo in the Golgi
Casler, J. C., Papanikou, E., Barrero, J. J., & Glick, B. S. (2019) · Journal of Cell Biology · doi:10.1083/jcb.201807195 · Cited by 77
Self-citationNoncytotoxic DsRed Derivatives for Whole-Cell Labeling
Strack, R. L., Keenan, R. J., & Glick, B. S. (2011) · Methods in Molecular Biology · doi:10.1007/978-1-61737-950-5_17 · Cited by 16
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Metadata
- Authors
- Strack, R. L., Hein, B., Bhattacharyya, D., Hell, S. W., Keenan, R. J., & Glick, B. S.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-08-12
- Volume
- 48
- Issue
- 35
- Pages
- 8279-8281
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2136133782
- Cited by
- 187
- References
- 1
- FWCI
- 6.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Strack, R. L., Hein, B., Bhattacharyya, D., Hell, S. W., Keenan, R. J., & Glick, B. S. (2009). A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell Labeling. Biochemistry. https://doi.org/10.1021/bi900870u
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