The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Spectroscopic Study of Extracellular Polymeric Substances from Bacillus subtilis: Aqueous Chemistry and Adsorption Effects
Abstract
Reactions at ionizable functional groups in extracellular polymeric substances (EPS) from Bacillus subtilis are found to affect aqueous phase conformation and adsorption to mineral surfaces. Characterization by HPSEC, XPS, and FTIR indicates a wide range in apparent molecular mass (0.57-128 kDa), with functional group composition depending on cell growth phase (exponential vs stationary) and location in suspension (free vs cell-bound). ATR-FTIR spectroscopy shows complexation and dissociation of protons on acidic functional groups that result in alpha-helical protein conformation at pH < 2.6 and random coil (unordered) conformation at higher pH (>6). EPS exhibit higher affinity for adsorption to alpha-FeOOH than amorphous SiO(2) because of surface charge effects. Increased amide II band intensity and an amide I band shift to higher frequency indicate changes in protein structure upon adsorption. Goethite-EPS spectra show emergent vibrations consistent with P-O-Fe bonding, which suggests a role of phosphodiester groups in the adsorption reaction.
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Nguyen, T., Roddick, F., & Fan, L. (2012) · Membranes · doi:10.3390/membranes2040804 · Cited by 802
Badireddy, A. R., Chellam, S., Gassman, P. L., Engelhard, M. H., Lea, A. S., & Rosso, K. M. (2010) · Water Research · doi:10.1016/j.watres.2010.06.024 · Cited by 538
Enhanced technology based for sewage sludge deep dewatering: A critical review
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Metadata
- Authors
- Omoike, A., & Chorover, J.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-04-23
- Volume
- 5
- Issue
- 4
- Pages
- 1219-1230
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2094409106
- Cited by
- 444
- References
- 54
- FWCI
- 5.17
- Type
- journal article
- Language
- en
- Open access
- closed
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