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Cited by 378

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol

Saha, Badal C., Iten, Loren B., Cotta, Michael A., Wu, Y. Victor·2008·Biotechnology Progress
DOI 10.1021/bp049564n
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Abstract

Rice hulls, a complex lignocellulosic material with high lignin (15.38 +/- 0.2%) and ash (18.71 +/- 0.01%) content, contain 35.62 +/- 0.12% cellulose and 11.96 +/- 0.73% hemicellulose and has the potential to serve as a low-cost feedstock for production of ethanol. Dilute H2SO4 pretreatments at varied temperature (120-190 degrees C) and enzymatic saccharification (45 degrees C, pH 5.0) were evaluated for conversion of rice hull cellulose and hemicellulose to monomeric sugars. The maximum yield of monomeric sugars from rice hulls (15%, w/v) by dilute H2SO4 (1.0%, v/v) pretreatment and enzymatic saccharification (45 degrees C, pH 5.0, 72 h) using cellulase, beta-glucosidase, xylanase, esterase, and Tween 20 was 287 +/- 3 mg/g (60% yield based on total carbohydrate content). Under this condition, no furfural and hydroxymethyl furfural were produced. The yield of ethanol per L by the mixed sugar utilizing recombinant Escherichia colistrain FBR 5 from rice hull hydrolyzate containing 43.6 +/- 3.0 g fermentable sugars (glucose, 18.2 +/- 1.4 g; xylose, 21.4 +/- 1.1 g; arabinose, 2.4 +/- 0.3 g; galactose, 1.6 +/- 0.2 g) was 18.7 +/- 0.6 g (0.43 +/- 0.02 g/g sugars obtained; 0.13 +/- 0.01 g/g rice hulls) at pH 6.5 and 35 degrees C. Detoxification of the acid- and enzyme-treated rice hull hydrolyzate by overliming (pH 10.5, 90 degrees C, 30 min) reduced the time required for maximum ethanol production (17 +/- 0.2 g from 42.0 +/- 0.7 g sugars per L) by the E. coli strain from 64 to 39 h in the case of separate hydrolysis and fermentation and increased the maximum ethanol yield (per L) from 7.1 +/- 2.3 g in 140 h to 9.1 +/- 0.7 g in 112 h in the case of simultaneous saccharification and fermentation.

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Research works printed but not linked: 30

  1. Saha, 1998, Fuel ethanol production from corn fiber: current status and technical prospects, Appl. Biochem. Biotechnol., 70-72, 115, 10.1007/BF02920129 · doi:10.1007/bf02920129
  2. Saha, 1999, Pretreatment and enzymatic saccharification of corn fiber, Appl. Biochem. Biotechnol., 76, 65, 10.1385/ABAB:76:2:65 · DOI · Paper page
  3. Bothast, 1999, Fermentation with new recombinant organisms, Biotechnol. Prog., 15, 867, 10.1021/bp990087w · DOI
  4. Watanabe, 1983, Structure of arabinoxylan of rice hull, Carbohydr. Res., 123, 83, 10.1016/0008-6215(83)88383-9 · doi:10.1016/0008-6215(83)88383-9
  5. Watanabe, 1984, Xyloglucans in cell walls of rice hull, Carbohydr. Res., 129, 229, 10.1016/0008-6215(84)85314-8 · doi:10.1016/0008-6215(84)85314-8
  6. Boateng, 1992, Fluidized-bed steam gassification of rice hull, Bioresour. Technol., 40, 235, 10.1016/0960-8524(92)90148-Q · doi:10.1016/0960-8524(92)90148-q
  7. Sharma, 2001, Hydrolysis of rice hull by cross-linked Aspergillus niger cellulase, Bioresour. Technol., 78, 281, 10.1016/S0960-8524(01)00010-4 · doi:10.1016/s0960-8524(01)00010-4
  8. Moniruzzaman, 1998, Ethanol production from dilute acid hydrolyzate of rice hulls using genetically engineered Escherichia coli, Biotechnol. Lett., 20, 943, 10.1023/A:1005490501736 · doi:10.1023/a:1005490501736
  9. Kumagai, 2004, Fractionation and saccharification of cellulose and hemicellulose in rice hull by hot-compressed-water treatment with two-step heating, J. Jpn. Inst. Energy, 83, 776, 10.3775/jie.83.776 · doi:10.3775/jie.83.776
  10. Dien, 2000, Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomass, Appl. Biochem. Biotechnol., 84-86, 181, 10.1385/ABAB:84-86:1-9:181 · DOI · Paper page
  11. Bothast, 1994, Fermentation of l-arabinose, d-xylose and d-glucose by ethanologenic Escherichia coli, Biotechnol. Lett., 16, 401, 10.1007/BF00245060 · DOI
  12. Juliano, 1985, Rice: Chemistry and Technology, 685
  13. Hugget, 1957, Glucose oxidase method for measurement of glucose, Biochem. J., 6, 12
  14. Saha, 2003, Hemicellulosebioconversion, J. Ind. Microbiol. Biotechnol., 30, 279, 10.1007/s10295-003-0049-x · DOI · Paper page
  15. Bothast, 1997, Ethanol production from agricultural biomass substrates, Adv. Appl. Microbiol., 44, 261, 10.1016/S0065-2164(08)70464-7 · DOI
  16. Lee, 1999, Dilute-acid hydrolysis of lignocellulosic biomass, Adv. Biochem. Eng. Biotechnol., 65, 93
  17. Saha, 2004, Lignocellulose Biodegradation, 2, 10.1021/bk-2004-0889.ch001 · DOI
  18. Kaar, 1998, Benefits from Tween during enzymic hydrolysis of corn stover, Biotechnol. Bioeng., 59, 419, 10.1002/(SICI)1097-0290(19980820)59:4<419::AID-BIT4>3.0.CO;2-J · DOI · Paper page
  19. Alkasrawi, 2003, The effect of Tween 20 on simultaneous saccharification and fermentation of softwood to ethanol, Enzyme Microb. Technol., 33, 71, 10.1016/S0141-0229(03)00087-5 · DOI · Paper page
  20. Mosier, 2001, Characterization of dicarboxylic acids for cellulose hydrolysis, Biotechnol. Prog., 17, 474, 10.1021/bp010028u · DOI
  21. Yamada, 1999, Rapid liquefaction of lignocellulosic waste by using ethylene carbonate, Bioresour. Technol., 70, 61, 10.1016/S0960-8524(99)00008-5 · DOI · Paper page
  22. Goldstein, 1983, Hydrolysis of cellulose with super concentrated hydrochloric-acid, Biotechnol. Bioeng., 13, 17
  23. Delgenes, 1990, Acid Hydrolysis of wheat straw and process considerations for ethanol fermentation by Pichia stipitis Y7124, Proc. Biochem., 25, 132
  24. Palmqvist, 2000, Fermentation of lignocellulosic hydrolyzates. I: inhibition and detoxification, Bioresour. Technol., 74, 17, 10.1016/S0960-8524(99)00160-1 · DOI · Paper page
  25. Palmqvist, 2000, Fermentation of lignocellulosic hydrolyzates. II: inhibitors and mechanism of inhibition, Bioresour. Technol., 74, 25, 10.1016/S0960-8524(99)00161-3 · DOI · Paper page
  26. Klinke, 2004, Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pretreatment of biomass, Appl. Microbiol. Biotechnol., 66, 10, 10.1007/s00253-004-1642-2 · DOI · Paper page
  27. Martinez, 2001, Detoxification of dilute acid hydrolyzates of lignocellulose with lime, Biotechnol. Prog., 17, 287, 10.1021/bp0001720 · DOI · Paper page
  28. Purwadi, 2004, Kinetic study of detoxification of dilute-acid hydrolyzates by Ca(OH)2, J. Biotechnol, 114, 187, 10.1016/j.jbiotec.2004.07.006 · doi:10.1016/j.jbiotec.2004.07.006
  29. Von Sivers, 1994, Cost analysis of ethanol production from willow using recombinant Escherichia coli, Biotechnol. Prog., 10, 555, 10.1021/bp00029a017 · DOI · Paper page
  30. Szczodrak, 1988, The Enzymatic hydrolysis and fermentation of pretreated wheat straw to ethanol, Biotechnol. Bioeng., 32, 771, 10.1002/bit.260320608 · DOI

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Metadata

Authors
Saha, B. C., Iten, L. B., Cotta, M. A., & Wu, Y. V.
Venue
Biotechnology Progress
Publisher
Wiley
Published
2008-09-05
Volume
21
Issue
3
Pages
816-822
ISSN
  • 1520-6033 (ISSN-L)
  • 8756-7938
DOI
10.1021/bp049564n
OpenAlex
W2073834721
Cited by
378
References
2
FWCI
8.19
Type
journal article
Language
en
Open access
closed
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 2552 d)

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CoChat. (2026). Citation check: Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol. https://app.cochat.ai/papers/10.1021/bp049564n
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Saha, B. C., Iten, L. B., Cotta, M. A., & Wu, Y. V. (2008). Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol. Biotechnology Progress. https://doi.org/10.1021/bp049564n

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