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Basic Concepts in Q Membrane Chromatography for Large-Scale Antibody Production
Abstract
The large-scale production of recombinant human monoclonal antibodies demands economical purification processes with high throughputs. In this article we briefly describe a common antibody process and evaluate the Q membrane adsorber for process-scale antibody production as an alternative to a Q-packed-bed column in a flow-through mode. The scientific concepts underlining Q membrane technology and its application are reviewed. The disadvantages and advantages of using Q membrane chromatography as a purification unit in large-scale production are discussed, including problems initially seen with the Q membrane scale-down model but solved with the invention of a new scale-down model. The new Q-membrane unit operation has a process capacity greater than 3,000 g/m(2) or 10.7 kg/L with a LRV over 5 for four model viruses. In this Review, a cost analysis illustrates that Q membrane chromatography is a viable alternative to Q column chromatography as a polishing step in a flow-through mode for process-scale antibody production.
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Citations
Cited by
Bioprocess membrane technology
van Reis, R., & Zydney, A. (2007) · Journal of Membrane Science · doi:10.1016/j.memsci.2007.02.045 · Cited by 773
Recent advances in large-scale production of monoclonal antibodies and related proteins
Shukla, A. A., & Thömmes, J. (2010) · Trends in Biotechnology · doi:10.1016/j.tibtech.2010.02.001 · Cited by 578
Recent advances in bioprocessing application of membrane chromatography
Orr, V., Zhong, L., Moo-Young, M., & Chou, C. P. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.01.007 · Cited by 253
Viral clearance for biopharmaceutical downstream processes
Shukla, A. A., & Aranha, H. (2015) · Pharmaceutical Bioprocessing · doi:10.4155/pbp.14.62 · Cited by 63
Metadata
- Authors
- Zhou, J. X., & Tressel, T.
- Venue
- Biotechnology Progress
- Publisher
- Wiley
- Published
- 2006-04-07
- Volume
- 22
- Issue
- 2
- Pages
- 341-349
- ISSN
- 1520-6033 (ISSN-L)
- 8756-7938
- OpenAlex
- W2153240158
- Cited by
- 225
- References
- 0
- FWCI
- 6.26
- Type
- journal article
- Language
- en
- Open access
- closed
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