The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Encapsulation of an Enzyme Cascade within the Bacteriophage P22 Virus-Like Particle
Abstract
Developing methods for investigating coupled enzyme systems under conditions that mimic the cellular environment remains a significant challenge. Here we describe a biomimetic approach for constructing densely packed and confined multienzyme systems through the co-encapsulation of 2 and 3 enzymes within a virus-like particle (VLP) that perform a coupled cascade of reactions, creating a synthetic metabolon. Enzymes are efficiently encapsulated in vivo with known stoichiometries, and the kinetic parameters of the individual and coupled activities are characterized. From the results we develop and validate a mathematical model for predicting the expected kinetics for coupled reactions under co-localized conditions.
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Citations
Cites
Macromolecular crowding: an important but neglected aspect of the intracellular environment
Ellis, R. J. (2001) · Current Opinion in Structural Biology · doi:10.1016/s0959-440x(00)00172-x · Cited by 1,153
Cited by
Enzymatic reactions in confined environments
Küchler, A., Yoshimoto, M., Luginbühl, S., Mavelli, F., & Walde, P. (2016) · Nature Nanotechnology · doi:10.1038/nnano.2016.54 · Cited by 733
Biocatalytic cascades operating on macromolecular scaffolds and in confined environments
Vázquez-González, M., Wang, C., & Willner, I. (2020) · Nature Catalysis · doi:10.1038/s41929-020-0433-1 · Cited by 293
Embracing Nature’s Catalysts: A Viewpoint on the Future of Biocatalysis
Hauer, B. (2020) · ACS Catalysis · doi:10.1021/acscatal.0c01708 · Cited by 284
Enzyme co-localisation: Mechanisms and benefits
Kondrat, S., Krauss, U., & von Lieres, E. (2022) · Current Research in Chemical Biology · doi:10.1016/j.crchbi.2022.100031 · Cited by 19
Metadata
- Authors
- Patterson, D. P., Schwarz, B., Waters, R. S., Gedeon, T., & Douglas, T.
- Venue
- ACS Chemical Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-12-06
- Volume
- 9
- Issue
- 2
- Pages
- 359-365
- ISSN
- 1554-8929 (ISSN-L)
- 1554-8937
- OpenAlex
- W2334151637
- Cited by
- 239
- References
- 1
- FWCI
- 7.93
- Type
- journal article
- Language
- en
- Open access
- closed
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