Bactericidal and Detoxification Effects of TiO2 Thin Film Photocatalysts

Abstract

To examine the special features of the antibacterial effect for a thin transparent titanium dioxide (TiO 2 ) film, the photocatalytic degradation of endotoxin, which is a pyrogenic constituent of Escherichia coli (E. coli), as well as its bactericidal activity, was investigated. The TiO 2 films were prepared from titanium isopropoxide solution, annealing at 500 °C. The bactericidal activity for E. coli cells was estimated by survival ratio calculated from the number of viable cells which form colonies on the nutrient agar plates. The endotoxin concentration was determined by the Limulus tests. When E. coli cells were killed by the TiO 2 photocatalyst under UV irradiation, the endotoxin from the cells was also degraded efficiently. This result shows that the TiO 2 photocatalyst has both bactericidal activity and decomposing activity for the endotoxin (i.e., detoxifying activity). The bactericidal effect of the TiO 2 thin film results from both inactivating the viability of the bacteria and the destruction of the E. coli cells. This feature renders TiO 2 photocatalysts to be applicable to environmental protections, especially in medical facilities where the endotoxin is needed to control.