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Methods to Explore Cellular Uptake of Ruthenium Complexes
Open accessAbstract
The cellular uptake of a series of dipyridophenazine (dppz) complexes of Ru(II) was examined by flow cytometry. The complexes, owing to their facile synthesis, stability, and luminescence, provide a route to compare and contrast systematically factors governing cellular entry. Substituting the ancillary ligands in the dppz complexes of Ru(II) permits variation in the overall complex charge, size, and hydrophobicity. In HeLa cells, cellular uptake appears to be facilitated by the lipophilic 4,7-diphenyl-1,10-phenanthroline (DIP) ligand. Despite the large size of Ru(DIP) 2 dppz 2+ (20 Å diameter), this complex is readily transported inside the cell compared to smaller and more hydrophilic complexes, such as Ru(bpy) 2 dppz 2+ . Accumulation in the cellular interior is confirmed by confocal microscopy.
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Lipinski, C. A., Lombardo, F., Dominy, B. W., & Feeney, P. J. (2001) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(00)00129-0 · Cited by 18,139
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Gill, M. R., & Thomas, J. A. (2012) · Chemical Society Reviews · doi:10.1039/c2cs15299a · Cited by 747
Luminescent Biological Probes Derived from Ruthenium(II) Estradiol Polypyridine Complexes
Lo, K. K.-W., Lee, T. K.-M., Lau, J. S.-Y., Poon, W.-L., & Cheng, S.-H. (2008) · Inorganic Chemistry · doi:10.1021/ic701735q · Cited by 306
Metadata
- Authors
- Puckett, C. A., & Barton, J. K.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-12-15
- Volume
- 129
- Issue
- 1
- Pages
- 46-47
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1969436685
- Cited by
- 467
- References
- 1
- FWCI
- 5.89
- Type
- journal article
- Language
- en
- Open access
- green
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