The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering
Abstract
Structural analysis of flexible macromolecular systems such as intrinsically disordered or multidomain proteins with flexible linkers is a difficult task as high-resolution techniques are barely applicable. A new approach, ensemble optimization method (EOM), is proposed to quantitatively characterize flexible proteins in solution using small-angle X-ray scattering (SAXS). The flexibility is taken into account by allowing for the coexistence of different conformations of the protein contributing to the experimental scattering pattern. These conformers are selected using a genetic algorithm from a pool containing a large number of randomly generated models covering the protein configurational space. Quantitative criteria are developed to analyze the EOM selected models and to determine the optimum number of conformers in the ensemble. Simultaneous fitting of multiple scattering patterns from deletion mutants, if available, provides yet more detailed local information about the structure. The efficiency of EOM is demonstrated in model and practical examples on completely or partially unfolded proteins and on multidomain proteins interconnected by linkers. In the latter case, EOM is able to distinguish between rigid and flexible proteins and to directly assess the interdomain contacts.
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Cites
Intrinsically unstructured proteins and their functions
Dyson, H. J., & Wright, P. E. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1589 · Cited by 3,871
Intrinsic Disorder and Protein Function
Dunker, A. K., Brown, C. J., Lawson, J. D., Iakoucheva, L. M., & Obradović, Z. (2002) · Biochemistry · doi:10.1021/bi012159+ · Cited by 1,731
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Metadata
- Authors
- Bernadó, P., Mylonas, E., Petoukhov, M. V., Blackledge, M., & Svergun, D. I.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-04-06
- Volume
- 129
- Issue
- 17
- Pages
- 5656-5664
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2072654958
- Cited by
- 1,189
- References
- 2
- FWCI
- 13.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bernadó, P., Mylonas, E., Petoukhov, M. V., Blackledge, M., & Svergun, D. I. (2007). Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering. Journal of the American Chemical Society. https://doi.org/10.1021/ja069124n
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