The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair
Open accessAbstract
We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/tRNA(CUA Pyl) pairs created in Escherichia coli can be used to expand the genetic code of Saccharomyces cerevisiae. In the process we have solved the key challenges of producing a functional tRNA(CUA Pyl) in yeast and discovered a pyrrolysyl-tRNA synthetase/tRNA(CUA Pyl) pair that is orthogonal in yeast. Using our approach we have incorporated an alkyne-containing amino acid for click chemistry, an important post-translationally modified amino acid and one of its analogs, a photocaged amino acid and a photo-cross-linking amino acid into proteins in yeast. Extensions of our approach will allow the growing list of useful amino acids that have been incorporated in E. coli with variant pyrrolysyl-tRNA synthetase/tRNA(CUA Pyl) pairs to be site-specifically incorporated into proteins in yeast.
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Cites
Regulation of cellular metabolism by protein lysine acetylation
Zhao, S., Xu, W., Jiang, W., Yu, W., Lin, Y., Zhang, T., Yao, J., Zhou, L., Zeng, Y., Li, H., et al. (2010) · Science · doi:10.1126/science.1179689 · Cited by 1,910
Self-citationUniversal rules and idiosyncratic features in tRNA identity
Giege, R., Sissler, M., & Florentz, C. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.22.5017 · Cited by 769
Cited by
Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
Lang, K., & Chin, J. W. (2014) · Chemical Reviews · doi:10.1021/cr400355w · Cited by 1,035
Self-citationExpanding and Reprogramming the Genetic Code of Cells and Animals
Chin, J. W. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035737 · Cited by 476
Self-citationDiazirine based photoaffinity labeling
Dubinsky, L., Krom, B. P., & Meijler, M. M. (2012) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2011.06.066 · Cited by 396
Expanding the Genetic Code of an Animal
Greiss, S., & Chin, J. W. (2011) · Journal of the American Chemical Society · doi:10.1021/ja2054034 · Cited by 254
Self-citationLabeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics
Tian, H., Fürstenberg, A., & Huber, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00084 · Cited by 114
Metadata
- Authors
- Hancock, S. M., Uprety, R., Deiters, A., & Chin, J. W.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-10-06
- Volume
- 132
- Issue
- 42
- Pages
- 14819-14824
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2028119145
- Cited by
- 220
- References
- 2
- FWCI
- 5.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hancock, S. M., Uprety, R., Deiters, A., & Chin, J. W. (2010). Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair. Journal of the American Chemical Society. https://doi.org/10.1021/ja104609m
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