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A One-Step Homogeneous Immunoassay for Cancer Biomarker Detection Using Gold Nanoparticle Probes Coupled with Dynamic Light Scattering
Open accessAbstract
A one-step homogeneous immunoassay for the detection of a prostate cancer biomarker, free-PSA (prostate specific antigen), was developed using gold nanoparticle probes coupled with dynamic light scattering (DLS) measurements. A spherical gold nanoparticle with a core diameter around 37 nm and a gold nanorod with a dimension of 40 by 10 nm were first conjugated with two different primary anti-PSA antibodies and then used as optical probes for the immunoassay. In the presence of antigen f-PSA in solution, the nanoparticles and nanorods aggregate together into pairs and oligomers through the formation of a sandwich type antibody-antigen-antibody linkage. The relative ratio of nanoparticle-nanorod pairs and oligomers versus individual nanoparticles was quantitatively monitored by DLS measurement. A correlation can be established between this relative ratio and the amount of antigen in solution. The light scattering intensity of nanoparticles and nanoparticle oligomers is several orders of magnitude higher than proteins and other typical molecules, making it possible to detect nanoparticle probes in the low picomolar concentration range. f-PSA in the concentration range from 0.1 to 10 ng/mL was detected by this one-step and washing-free homogeneous immunoassay.
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Cited by
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Huang, X., Neretina, S., & El‐Sayed, M. A. (2009) · Advanced Materials · doi:10.1002/adma.200802789 · Cited by 1,921
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Zhang, S., Gao, H., & Bao, G. (2015) · ACS Nano · doi:10.1021/acsnano.5b03184 · Cited by 1,103
Colorimetric Detection of Pb2+ Using Glutathione Functionalized Gold Nanoparticles
Chai, F., Wang, C., Wang, T., Li, L., & Su, Z. (2010) · ACS Applied Materials & Interfaces · doi:10.1021/am100107k · Cited by 375
Stress Biomarkers in Biological Fluids and Their Point-of-Use Detection
Steckl, A. J., & Ray, P. (2018) · ACS Sensors · doi:10.1021/acssensors.8b00726 · Cited by 319
Physicochemical characterization of drug nanocarriers
Berbel Manaia, E., Paiva Abuçafy, M., Chiari-Andréo, B. G., Lallo Silva, B., Oshiro-Júnior, J. A., & Chiavacci, L. (2017) · International Journal of Nanomedicine · doi:10.2147/ijn.s133832 · Cited by 207
Metadata
- Authors
- Liu, X., Dai, Q., Austin, L., Coutts, J., Knowles, G., Zou, J., Chen, H., & Huo, Q.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-02-08
- Volume
- 130
- Issue
- 9
- Pages
- 2780-2782
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2038665562
- Cited by
- 675
- References
- 0
- FWCI
- 19.50
- Type
- journal article
- Language
- en
- Open access
- green
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- Liu, X., Dai, Q., Austin, L., Coutts, J., Knowles, G., Zou, J., Chen, H., & Huo, Q. (2008). A One-Step Homogeneous Immunoassay for Cancer Biomarker Detection Using Gold Nanoparticle Probes Coupled with Dynamic Light Scattering. Journal of the American Chemical Society. https://doi.org/10.1021/ja711298b
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