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Temperature-Regulated Activity of Responsive Polymer−Protein Conjugates Prepared by Grafting-from via RAFT Polymerization
Abstract
A facile route to well-defined "smart" polymer-protein conjugates with tunable bioactivity is reported. Protein modification with a reversible addition-fragmentation chain transfer (RAFT) agent and subsequent room temperature polymerization in aqueous media led to conjugates of poly(N-isopropylacrylamide) and a model protein. Representing the first example of polymer-protein conjugation with RAFT agent immobilization via the "R-group" approach, high molecular weight and reductively stable conjugates were accessible without extensive purification or adverse effects on the protein structure. An increase in molecular weight with conversion was observed for the chains grafted from the protein surface, confirming the controlled nature of the polymerization. The responsive behavior of the immobilized polymer facilitated conjugate isolation and also allowed environmental modulation of bioactivity.
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Cited by
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Pelegri-O’Day, E. M., Lin, E.-W., & Maynard, H. D. (2014) · Journal of the American Chemical Society · doi:10.1021/ja504390x · Cited by 622
Gregory, A., & Stenzel, M. H. (2012) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2011.08.004 · Cited by 470
Roy, D., Cambre, J. N., & Sumerlin, B. S. (2009) · Chemical Communications · doi:10.1039/b900374f · Cited by 264
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Metadata
- Authors
- De, P., Li, M., Gondi, S. R., & Sumerlin, B. S.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-07-30
- Volume
- 130
- Issue
- 34
- Pages
- 11288-11289
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2087060219
- Cited by
- 404
- References
- 0
- FWCI
- 16.84
- Type
- journal article
- Language
- en
- Open access
- closed
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