The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteins via a Pyrrolysyl-tRNA Synthetase/tRNACUA Pair and Click Chemistry
Abstract
We demonstrate that an orthogonal Methanosarcina barkeri MS pyrrolysyl-tRNA synthetase/tRNA(CUA) pair directs the efficient, site-specific incorporation of N6-[(2-propynyloxy)carbonyl]-L-lysine, containing a carbon-carbon triple bond, and N6-[(2-azidoethoxy)carbonyl]-L-lysine, containing an azido group, into recombinant proteins in Escherichia coli. Proteins containing the alkyne functional group are labeled with an azido biotin and an azido fluorophore, via copper catalyzed [3+2] cycloaddition reactions, to produce the corresponding triazoles in good yield. The methods reported are useful for the site-specific labeling of recombinant proteins and may be combined with mutually orthogonal methods of introducing unnatural amino acids into proteins as well as with chemically orthogonal methods of protein labeling. This should allow the site specific incorporation of multiple distinct probes into proteins and the control of protein topology and structure by intramolecular orthogonal conjugation reactions.
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An Expanded Eukaryotic Genetic Code
Chin, J. W., Cropp, T. A., Anderson, J. C., Mukherji, M., Zhang, Z., & Schultz, P. G. (2003) · Science · doi:10.1126/science.1084772 · Cited by 820
Self-citationAddition of p-Azido-l-phenylalanine to the Genetic Code of Escherichia coli
Chin, J. W., Santoro, S. W., Martin, A. B., King, D. S., Wang, L., & Schultz, P. G. (2002) · Journal of the American Chemical Society · doi:10.1021/ja027007w · Cited by 721
Self-citationGenetically encoding Nε-acetyllysine in recombinant proteins
Neumann, H., Peak-Chew, S. Y., & Chin, J. W. (2008) · Nature Chemical Biology · doi:10.1038/nchembio.73 · Cited by 592
Self-citationA chemical toolkit for proteins — an expanded genetic code
Xie, J., & Schultz, P. G. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2005 · Cited by 408
Yanagisawa, T., Ishii, R., Fukunaga, R., Kobayashi, T., Sakamoto, K., & Yokoyama, S. (2008) · Chemistry & Biology · doi:10.1016/j.chembiol.2008.10.004 · Cited by 336
Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion
Wang, K., Neumann, H., Peak-Chew, S. Y., & Chin, J. W. (2007) · Nature Biotechnology · doi:10.1038/nbt1314 · Cited by 312
Self-citationMukai, T., Kobayashi, T., Hino, N., Yanagisawa, T., Sakamoto, K., & Yokoyama, S. (2008) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2008.04.164 · Cited by 279
A New Strategy for the Site-Specific Modification of Proteins in Vivo
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Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome
Neumann, H., Wang, K., Davis, L., Garcia-Alai, M., & Chin, J. W. (2010) · Nature · doi:10.1038/nature08817 · Cited by 622
Self-citationExpanding and Reprogramming the Genetic Code of Cells and Animals
Chin, J. W. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035737 · Cited by 476
Self-citationSchmied, W. H., Elsässer, S. J., Uttamapinant, C., & Chin, J. W. (2014) · Journal of the American Chemical Society · doi:10.1021/ja5069728 · Cited by 270
Self-citationLabeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics
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Metadata
- Authors
- Nguyen, D. P., Lusic, H., Neumann, H., Kapadnis, P. B., Deiters, A., & Chin, J. W.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-06-10
- Volume
- 131
- Issue
- 25
- Pages
- 8720-8721
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2067826785
- Cited by
- 317
- References
- 15
- FWCI
- 13.09
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Nguyen, D. P., Lusic, H., Neumann, H., Kapadnis, P. B., Deiters, A., & Chin, J. W. (2009). Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteins via a Pyrrolysyl-tRNA Synthetase/tRNACUA Pair and Click Chemistry. Journal of the American Chemical Society. https://doi.org/10.1021/ja900553w
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