The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity
Open accessAbstract
The unique reactivity of two sortase enzymes, SrtA(staph) from Staphylococcus aureus and SrtA(strep) from Streptococcus pyogenes, is exploited for site-specific labeling of a single polypeptide with different labels at its N and C termini. SrtA(strep) is used to label the protein's C terminus at an LPXTG site with a fluorescently labeled dialanine nucleophile. Selective N-terminal labeling of proteins containing N-terminal glycine residues is achieved using SrtA(staph) and LPXT derivatives. The generality of N-terminal labeling with SrtA(staph) is demonstrated by near-quantitative labeling of multiple protein substrates with excellent site specificity.
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Mazmanian, S. K., Ton-That, H., Su, K., & Schneewind, O. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.032523999 · Cited by 372
An embarrassment of sortases – a richness of substrates?
Pallen, M. J., Lam, A. C., Antonio, M., & Dunbar, K. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(01)01956-4 · Cited by 225
Surface Protein IsdC and Sortase B Are Required for Heme-Iron Scavenging of Bacillus anthracis
Maresso, A. W., Chapa, T. J., & Schneewind, O. (2006) · Journal of Bacteriology · doi:10.1128/jb.01011-06 · Cited by 135
Cited by
Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
Lang, K., & Chin, J. W. (2014) · Chemical Reviews · doi:10.1021/cr400355w · Cited by 1,035
Contemporary approaches to site-selective protein modification
Hoyt, E. A., Cal, P. M. S. D., Oliveira, B. L., & Bernardes, G. J. L. (2019) · Nature Reviews Chemistry · doi:10.1038/s41570-019-0079-1 · Cited by 537
Targeting the N terminus for site-selective protein modification
Rosen, C. B., & Francis, M. B. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2416 · Cited by 389
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Guimaraes, C. P., Witte, M. D., Theile, C. S., Bozkurt, G., Kundrat, L., Blom, A. E. M., & Ploegh, H. L. (2013) · Nature Protocols · doi:10.1038/nprot.2013.101 · Cited by 345
Self-citationStructural basis for ubiquitin-mediated antiviral signal activation by RIG-I
Peisley, A., Wu, B., Xu, H., Chen, Z. J., & Hur, S. (2014) · Nature · doi:10.1038/nature13140 · Cited by 316
Metadata
- Authors
- Antos, J. M., Chew, G.-L., Guimaraes, C. P., Yoder, N. C., Grotenbreg, G. M., Popp, M. W.-L., & Ploegh, H. L.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-07-17
- Volume
- 131
- Issue
- 31
- Pages
- 10800-10801
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2024669426
- Cited by
- 241
- References
- 3
- FWCI
- 3.96
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Antos, J. M., Chew, G.-L., Guimaraes, C. P., Yoder, N. C., Grotenbreg, G. M., Popp, M. W.-L., & Ploegh, H. L. (2009). Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity. Journal of the American Chemical Society. https://doi.org/10.1021/ja902681k
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