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Echinacea Standardization: Analytical Methods for Phenolic Compounds and Typical Levels in Medicinal Species
Abstract
A proposed standard extraction and HPLC analysis method has been used to measure typical levels of various phenolic compounds in the medicinally used Echinacea species. Chicoric acid was the main phenolic in E. purpurea roots (mean 2.27% summer, 1.68% autumn) and tops (2.02% summer, 0.52% autumn), and echinacoside was the main phenolic in E. angustifolia (1.04%) and E. pallida roots (0.34%). Caftaric acid was the other main phenolic compound in E. purpurea roots (0.40% summer, 0.35% autumn) and tops (0.82% summer, 0.18% autumn), and cynarin was a characteristic component of E. angustifolia roots (0.12%). Enzymatic browning during extraction could reduce the measured levels of phenolic compounds by >50%. Colorimetric analyses for total phenolics correlated well with the HPLC results for E. purpurea and E. angustifolia, but the colorimetric method gave higher values.
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Citations
Cited by
Analysis of Antioxidative Phenolic Compounds in Artichoke (Cynara scolymus L.)
Wang, M., Simon, J. E., Aviles, I. F., He, K., Zheng, Q.-Y., & Tadmor, Y. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020792b · Cited by 440
Analysis of phenolic compounds and radical scavenging activity of Echinacea spp.
Pellati, F., Benvenuti, S., Magro, L., Melegari, M., & Soragni, F. (2004) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/s0731-7085(03)00645-9 · Cited by 306
Variation of phenolic content in coconut oil extracted by two conventional methods
Seneviratne, K. N., & Sudarshana Dissanayake, D. M. (2008) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2006.01493.x · Cited by 124
Metadata
- Authors
- Perry, N. B., Burgess, E. J., & Glennie, V. L.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-03-03
- Volume
- 49
- Issue
- 4
- Pages
- 1702-1706
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1996025880
- Cited by
- 161
- References
- 0
- FWCI
- 16.97
- Type
- journal article
- Language
- en
- Open access
- closed
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- Perry, N. B., Burgess, E. J., & Glennie, V. L. (2001). Echinacea Standardization: Analytical Methods for Phenolic Compounds and Typical Levels in Medicinal Species. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf001331y
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