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Identification and Quantification of Polyphenols in Carob Fruits (Ceratonia siliqua L.) and Derived Products by HPLC-UV-ESI/MSn
Abstract
The polyphenolic patterns of carob pods (Ceratonia siliqua L.) and derived products were identified and quantified using high-performance liquid chromatography-UV absorption-electrospray ion trap mass spectrometry after pressurized liquid extraction and solid-phase extraction. In carob fiber, 41 individual phenolic compounds could be identified. In addition, spectrophotometric quantification using the Folin-Ciocalteu and vanillin assays was performed, and the antioxidative activity was determined as the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Carob pods contain 448 mg/kg extractable polyphenols comprising gallic acid, hydrolyzable and condensed tannins, flavonol-glycosides, and traces of isoflavonoids. Among the products investigated, carob fiber, a carob pod preparation rich in insoluble dietary fiber (total polyphenol content = 4142 mg/kg), shows the highest concentrations in flavonol-glycosides and hydrolyzable tannins, whereas roasted carob products contain the highest levels of gallic acid. The production process seems to have an important influence on the polyphenolic patterns and quantities in carob products.
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Cited by
Unravelling the conundrum of tannins in animal nutrition and health
Mueller‐Harvey, I. (2006) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2577 · Cited by 965
Quiles-Carrillo, L., Mellinas, C., Garrigos, M. C., Balart, R., & Torres-Giner, S. (2019) · Food Analytical Methods · doi:10.1007/s12161-019-01596-3 · Cited by 59
d’Unienville, N. M. A., Blake, H. T., Coates, A. M., Hill, A. M., Nelson, M. J., & Buckley, J. D. (2021) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-021-00472-y · Cited by 56
Metadata
- Authors
- Papagiannopoulos, M., Wollseifen, H. R., Mellenthin, A., Haber, B., & Galensa, R.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-05-15
- Volume
- 52
- Issue
- 12
- Pages
- 3784-3791
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2037615866
- Cited by
- 237
- References
- 0
- FWCI
- 3.48
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Identification and Quantification of Polyphenols in Carob Fruits (Ceratonia siliqua L.) and Derived Products by HPLC-UV-ESI/MSn. https://app.cochat.ai/papers/10.1021/jf030660y
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- Papagiannopoulos, M., Wollseifen, H. R., Mellenthin, A., Haber, B., & Galensa, R. (2004). Identification and Quantification of Polyphenols in Carob Fruits (Ceratonia siliqua L.) and Derived Products by HPLC-UV-ESI/MSn. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf030660y
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