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Identification and Quantification of Polyphenols in Carob Fruits (Ceratonia siliqua L.) and Derived Products by HPLC-UV-ESI/MSn

Papagiannopoulos, Menelaos, Wollseifen, Hans Rainer, Mellenthin, Annett, Haber, Bernd, Galensa, Rudolf·2004·Journal of Agricultural and Food Chemistry

Abstract

The polyphenolic patterns of carob pods (Ceratonia siliqua L.) and derived products were identified and quantified using high-performance liquid chromatography-UV absorption-electrospray ion trap mass spectrometry after pressurized liquid extraction and solid-phase extraction. In carob fiber, 41 individual phenolic compounds could be identified. In addition, spectrophotometric quantification using the Folin-Ciocalteu and vanillin assays was performed, and the antioxidative activity was determined as the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Carob pods contain 448 mg/kg extractable polyphenols comprising gallic acid, hydrolyzable and condensed tannins, flavonol-glycosides, and traces of isoflavonoids. Among the products investigated, carob fiber, a carob pod preparation rich in insoluble dietary fiber (total polyphenol content = 4142 mg/kg), shows the highest concentrations in flavonol-glycosides and hydrolyzable tannins, whereas roasted carob products contain the highest levels of gallic acid. The production process seems to have an important influence on the polyphenolic patterns and quantities in carob products.

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Metadata

Authors
Papagiannopoulos, M., Wollseifen, H. R., Mellenthin, A., Haber, B., & Galensa, R.
Venue
Journal of Agricultural and Food Chemistry
Published
2004-05-15
Volume
52
Issue
12
Pages
3784-3791
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2037615866
Cited by
237
References
0
FWCI
3.48
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Identification and Quantification of Polyphenols in Carob Fruits (Ceratonia siliqua L.) and Derived Products by HPLC-UV-ESI/MSn. https://app.cochat.ai/papers/10.1021/jf030660y
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Papagiannopoulos, M., Wollseifen, H. R., Mellenthin, A., Haber, B., & Galensa, R. (2004). Identification and Quantification of Polyphenols in Carob Fruits (Ceratonia siliqua L.) and Derived Products by HPLC-UV-ESI/MSn. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf030660y

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