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Rapid Determination of Sugars, Nonvolatile Acids, and Ascorbic Acid in Strawberry and Other Fruits

Pérez, Ana G., Olías, Raquel, Espada, Javier, Olías, José M., Sanz, Carlos·1997·Journal of Agricultural and Food Chemistry

Abstract

An analytical procedure to determine major sugars and organic acids, including vitamin C, in fruits was developed using a C 18 Sep-Pak cleanup process and an ion exclusion HPLC column. Dual UV monitoring and refractive index were performed for detection. To attain optimal separation and quantitation, 0.0085N H 2 SO 4 was used as the mobile phase and the column temperature was maintained at 23 °C. This procedure was compared to others for the individual quantitation of sugars, organic acids, and vitamin C. Recovery and reproducibility of this analytical procedure were quite acceptable for strawberry and four other common fruits, allowing the analysis of all the components using a single-injection HPLC analysis in <22 min. Keywords: Simultaneous analysis; high-performance liquid chromatography; sugars; organic acids; vitamin C; strawberry; fruits

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Metadata

Authors
Pérez, A. G., Olías, R., Espada, J., Olías, J. M., & Sanz, C.
Venue
Journal of Agricultural and Food Chemistry
Published
1997-09-01
Volume
45
Issue
9
Pages
3545-3549
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2047972633
Cited by
179
References
0
FWCI
6.87
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Rapid Determination of Sugars, Nonvolatile Acids, and Ascorbic Acid in Strawberry and Other Fruits. https://app.cochat.ai/papers/10.1021/jf9701704
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Pérez, A. G., Olías, R., Espada, J., Olías, J. M., & Sanz, C. (1997). Rapid Determination of Sugars, Nonvolatile Acids, and Ascorbic Acid in Strawberry and Other Fruits. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf9701704

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