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Cited by 337

The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.

A New Type of Flavor Precursors in Vitisvinifera L. cv. Sauvignon Blanc:S-Cysteine Conjugates

Tominaga, Takatoshi, Peyrot des Gachons, Catherine, Dubourdieu, Denis·1998·Journal of Agricultural and Food Chemistry

Abstract

Three flavor-active volatile thiols (4-mercapto-4-methylpentan-2-one, 4-mercapto-4-methylpentan-2-ol and 3-mercaptohexan-1-ol) involved in Vitis vinifera L. var. Sauvignon blanc wine aroma can be generated in vitro from nonvolatile must extracts by the enzyme action of a cell-free extract obtained from the gastrointestinal bacterium Eubacterium limosum . The specificity of a cysteine conjugate β-lyase (EC 4.4.1.13) which is contained in the cell-free extract, strongly suggested the existence of precursor for these volatile thiols having a structure of S -cysteine conjugate. Gas-phase chromatography analysis coupled with mass spectrometry of must extract in the form of trimethylsilylated derivatives, verified this hypothesis. The release of flavor-active volatile thiols during alcoholic fermentation of the must is shown to be due to the degradation, by yeast, of the corresponding S -cysteine conjugates. This mechanism explains the amplification of the typical Sauvignon blanc grape aroma during alcoholic fermentation. Keywords: Cysteine conjugate β- lyase; Eubacterium limosum; flavor precursor; metal ion chelated column; Sauvignon blanc; S -cysteine conjugate; varietal aroma

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Metadata

Authors
Tominaga, T., Peyrot des Gachons, C., & Dubourdieu, D.
Venue
Journal of Agricultural and Food Chemistry
Published
1998-11-06
Volume
46
Issue
12
Pages
5215-5219
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2054631925
Cited by
337
References
13
FWCI
5.10
Type
journal article
Language
en
Open access
closed

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