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Patterning Mammalian Cells Using Elastomeric Membranes
Abstract
We describe the patterning of proteins and cells onto the surfaces of bacteriological Petri dishes, glass, and poly(dimethylsiloxane) (PDMS) with the use of elastomeric lift-off membranes free-standing polymer films that have circular or square holes with diameters, sides, and height ≥50 μm. Cells are patterned within the physical constraints provided by the holes of the membranes; these constraints can be released to allow the cells to spread onto the rest of the surface or to remain in the pattern by controlling the properties of the surfaces. Careful control of the properties of the surfaces of the substrates are required to cause the cells to adhere to the substrate and not to the membrane, and to avoid damage to the cells on removing the membrane. This strategy of membrane-based patterning given the acronym MEMPAT for brevity offers a more convenient way for patterning cells on surfaces and for studying cell spreading than existing methods.
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Citations
Cited by
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Sun, K., Xie, Y., Ye, D., Zhao, Y., Cui, Y., Long, F., Zhang, W., & Jiang, X. (2012) · Langmuir · doi:10.1021/la2041967 · Cited by 87
Vacuum-assisted cell loading enables shear-free mammalian microfluidic culture
Kolnik, M., Tsimring, L. S., & Hasty, J. (2012) · Lab on a Chip · doi:10.1039/c2lc40569e · Cited by 86
Sun, K., Song, L., Xie, Y., Liu, D., Wang, D., Wang, Z., Ma, W., Zhu, J., & Jiang, X. (2011) · Langmuir · doi:10.1021/la2012099 · Cited by 51
Metadata
- Authors
- Ostuni, E., Kane, R., Chen, C. S., Ingber, D. E., & Whitesides, G. M.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-08-30
- Volume
- 16
- Issue
- 20
- Pages
- 7811-7819
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W1966962106
- Cited by
- 316
- References
- 0
- FWCI
- 5.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ostuni, E., Kane, R., Chen, C. S., Ingber, D. E., & Whitesides, G. M. (2000). Patterning Mammalian Cells Using Elastomeric Membranes. Langmuir. https://doi.org/10.1021/la000382m
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