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Selective Deposition of Proteins and Cells in Arrays of Microwells
Abstract
This paper describes a method for depositing proteins and cells selectively into microwells fabricated in poly(dimethylsiloxane) an elastomeric organic polymer. The arrays of microwells were generated using soft lithography. The surface between the wells was coated with bovine serum albumin, and the surface within the wells was coated with fibronectin (FN) an adhesive extracellular matrix protein. Fluorescence staining of FN using antibodies indicated that the protein had adsorbed to the wells selectively. Exposure of the FN-coated wells to a suspension of bovine capillary endothelial cells resulted in attachment and spreading of the cells only in the wells. This method for patterning cells was demonstrated with wells of several diameters (≤100 μm) and depths (≤50 μm). It should be useful for (i) the development of sensors based on arrays of cells, (ii) screening combinatorial libraries of ligands on cells, and (iii) testing the effect on cells of compounds and samples relevant to problems in environmental chemistry, pharmacology, medicinal chemistry, research biology, and biodefense.
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Cited by
Soft Lithography in Biology and Biochemistry
Whitesides, G. M., Ostuni, E., Takayama, S., Jiang, X., & Ingber, D. E. (2001) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.3.1.335 · Cited by 2,607
Self-citationSuperhydrophobic surfaces: from structural control to functional application
Zhang, X., Shi, F., Niu, J., Jiang, Y., & Wang, Z. (2008) · J. Mater. Chem. · doi:10.1039/b711226b · Cited by 1,768
Campisi, M., Shin, Y., Osaki, T., Hajal, C., Chiono, V., & Kamm, R. D. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.07.014 · Cited by 718
Dynamic single cell culture array
Carlo, D. D., Wu, L. Y., & Lee, L. P. (2006) · Lab on a Chip · doi:10.1039/b605937f · Cited by 707
Quan, P.-L., Sauzade, M., & Brouzes, E. (2018) · Sensors · doi:10.3390/s18041271 · Cited by 664
Metadata
- Authors
- Ostuni, E., Chen, C. S., Ingber, D. E., & Whitesides, G. M.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-04-05
- Volume
- 17
- Issue
- 9
- Pages
- 2828-2834
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W1965177733
- Cited by
- 232
- References
- 0
- FWCI
- 2.80
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ostuni, E., Chen, C. S., Ingber, D. E., & Whitesides, G. M. (2001). Selective Deposition of Proteins and Cells in Arrays of Microwells. Langmuir. https://doi.org/10.1021/la001372o
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
