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Gold Nanoparticle−Cytochrome c Complexes: The Effect of Nanoparticle Ligand Charge on Protein Structure
Abstract
We report the effect of nanoparticle ligand charge on the structure of a covalently, site-specifically linked protein. Au nanoparticles with positive, negative, and neutral ligands were appended to a specific cysteine, C102, of Saccharomyces cerevisiae cytochrome c. Conjugates were purified by HPLC or gel electrophoresis. Circular dichroism spectroscopy shows that changing the nanoparticle ligand dramatically influences the attached cytochrome c structure. The protein retains its structure with neutral ligands but denatures in the presence of charged species. This is rationalized by the electrostatic interaction of amino acids in the local vicinity of C102 with the endgroups of the ligand.
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Cited by
Walkey, C. D., & Chan, W. C. W. (2012) · Chem. Soc. Rev. · doi:10.1039/c1cs15233e · Cited by 1,758
Treuel, L., Brandholt, S., Maffre, P., Wiegele, S., Shang, L., & Nienhaus, G. U. (2014) · ACS Nano · doi:10.1021/nn405019v · Cited by 419
The Role of Surface Functionality in Determining Nanoparticle Cytotoxicity
Kim, S. T., Saha, K., Kim, C., & Rotello, V. M. (2013) · Accounts of Chemical Research · doi:10.1021/ar3000647 · Cited by 403
Metadata
- Authors
- Aubin-Tam, M.-E., & Hamad-Schifferli, K.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-11-12
- Volume
- 21
- Issue
- 26
- Pages
- 12080-12084
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2024607041
- Cited by
- 228
- References
- 0
- FWCI
- 3.64
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
