The DOI matches its Crossref record.
Application of Thiolated Gold Nanoparticles for the Enhancement of Glucose Oxidase Activity
Abstract
Glucose oxidase (GOx) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles (5-8 nm) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lower value of the Michaelis-Menton constant obtained for the immobilized (3.74 mM) GOx compared with that for the free (5.85 mM) GOx suggests significant enhancement in the activity of GOx attached to thiolated gold nanoparticles. The covalently immobilized GOx thiolated nanoparticles exhibit a response time of 30 s, a shelf life of more than 6 months, and improved tolerance to both pH and temperature.
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Cited by
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Ansari, S. A., & Husain, Q. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.09.005 · Cited by 1,118
Nanoparticles for biomedical imaging
Nune, S. K., Gunda, P., Thallapally, P. K., Lin, Y.-Y., Laird Forrest, M., & Berkland, C. J. (2009) · Expert Opinion on Drug Delivery · doi:10.1517/17425240903229031 · Cited by 450
Conde, J., Dias, J. T., Grazú, V., Moros, M., Baptista, P. V., & de la Fuente, J. M. (2014) · Frontiers in Chemistry · doi:10.3389/fchem.2014.00048 · Cited by 425
Nanomaterials for bio-functionalized electrodes: recent trends
Walcarius, A., Minteer, S. D., Wang, J., Lin, Y., & Merkoçi, A. (2013) · Journal of Materials Chemistry B · doi:10.1039/c3tb20881h · Cited by 366
Wu, P., He, Y., Wang, H.-F., & Yan, X.-P. (2010) · Analytical Chemistry · doi:10.1021/ac902531g · Cited by 345
Waheed, A., Mansha, M., & Ullah, N. (2018) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2018.04.012 · Cited by 305
Arya, S. K., Dey, A., & Bhansali, S. (2011) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2011.07.015 · Cited by 118
Self-citationStrategies of enzyme immobilization on nanomatrix supports and their intracellular delivery
Sharifi, M., Karim, A. Y., Mustafa Qadir Nanakali, N., Salihi, A., Aziz, F. M., Hong, J., Khan, R. H., Saboury, A. A., Hasan, A., Abou-Zied, O. K., et al. (2020) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2019.1643787 · Cited by 29
Metadata
- Authors
- Pandey, P., Singh, S. P., Arya, S. K., Gupta, V., Datta, M., Singh, S., & Malhotra, B. D.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-01-30
- Volume
- 23
- Issue
- 6
- Pages
- 3333-3337
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2058123640
- Cited by
- 242
- References
- 0
- FWCI
- 7.54
- Type
- journal article
- Language
- en
- Open access
- closed
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