The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Surface Functionalization of Silica Nanoparticles Supports Colloidal Stability in Physiological Media and Facilitates Internalization in Cells
Abstract
The influence of the surface functionalization of silica particles on their colloidal stability in physiological media is studied and correlated with their uptake in cells. The surface of 55 ± 2 nm diameter silica particles is functionalized by amino acids or amino- or poly(ethylene glycol) (PEG)-terminated alkoxysilanes to adjust the zeta potential from highly negative to positive values in ethanol. A transfer of the particles into water, physiological buffers, and cell culture media reduces the absolute value of the zeta potential and changes the colloidal stability. Particles stabilized by L-arginine, L-lysine, and amino silanes with short alkyl chains are only moderately stable in water and partially in PBS or TRIS buffer, but aggregate in cell culture media. Nonfunctionalized, N-(6-aminohexyl)-3-aminopropyltrimethoxy silane (AHAPS), and PEG-functionalized particles are stable in all media under study. The high colloidal stability of positively charged AHAPS-functionalized particles scales with the ionic strength of the media, indicating a mainly electrostatical stabilization. PEG-functionalized particles show, independently from the ionic strength, no or only minor aggregation due to additional steric stabilization. AHAPS stabilized particles are readily taken up by HeLa cells, likely as the positive zeta potential enhances the association with the negatively charged cell membrane. Positively charged particles stabilized by short alkyl chain aminosilanes adsorb on the cell membrane, but are weakly taken up, since aggregation inhibits their transport. Nonfunctionalized particles are barely taken up and PEG-stabilized particles are not taken up at all into HeLa cells, despite their high colloidal stability. The results indicate that a high colloidal stability of nanoparticles combined with an initial charge-driven adsorption on the cell membrane is essential for efficient cellular uptake.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Brunner, T. J., Wick, P., Manser, P., Spohn, P., Grass, R. N., Limbach, L. K., Bruinink, A., & Stark, W. J. (2006) · Environmental Science & Technology · doi:10.1021/es052069i · Cited by 1,385
Limbach, L. K., Li, Y., Grass, R. N., Brunner, T. J., Hintermann, M. A., Muller, M., Gunther, D., & Stark, W. J. (2005) · Environmental Science & Technology · doi:10.1021/es051043o · Cited by 779
Cited by
Nanoparticle colloidal stability in cell culture media and impact on cellular interactions
Moore, T. L., Rodriguez-Lorenzo, L., Hirsch, V., Balog, S., Urban, D., Jud, C., Rothen-Rutishauser, B., Lattuada, M., & Petri-Fink, A. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00487f · Cited by 1,082
Rancan, F., Gao, Q., Graf, C., Troppens, S., Hadam, S., Hackbarth, S., Kembuan, C., Blume-Peytavi, U., Rühl, E., Lademann, J., et al. (2012) · ACS Nano · doi:10.1021/nn301622h · Cited by 288
Self-citationKurtz-Chalot, A., Villiers, C., Pourchez, J., Boudard, D., Martini, M., Marche, P. N., Cottier, M., & Forest, V. (2017) · Materials Science and Engineering: C · doi:10.1016/j.msec.2017.02.028 · Cited by 102
Engineered nanoparticles for imaging and drug delivery in colorectal cancer
Pavitra, E., Dariya, B., Srivani, G., Kang, S.-M., Alam, A., Sudhir, P.-R., Kamal, M. A., Raju, G. S. R., Han, Y.-K., Lakkakula, B. V. K. S., et al. (2021) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2019.06.017 · Cited by 102
Metadata
- Authors
- Graf, C., Gao, Q., Schütz, I., Noufele, C. N., Ruan, W., Posselt, U., Korotianskiy, E., Nordmeyer, D., Rancan, F., Hadam, S., Vogt, A., Lademann, J., Haucke, V., & Rühl, E.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-05-07
- Volume
- 28
- Issue
- 20
- Pages
- 7598-7613
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2004666884
- Cited by
- 220
- References
- 2
- FWCI
- 6.49
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Surface Functionalization of Silica Nanoparticles Supports Colloidal Stability in Physiological Media and Facilitates Internalization in Cells. https://app.cochat.ai/papers/10.1021/la204913t
- The paper
- Graf, C., Gao, Q., Schütz, I., Noufele, C. N., Ruan, W., Posselt, U., Korotianskiy, E., Nordmeyer, D., Rancan, F., Hadam, S., Vogt, A., Lademann, J., Haucke, V., & Rühl, E. (2012). Surface Functionalization of Silica Nanoparticles Supports Colloidal Stability in Physiological Media and Facilitates Internalization in Cells. Langmuir. https://doi.org/10.1021/la204913t
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
