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Cited by 530

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Attaching Proteins to Carbon Nanotubes via Diimide-Activated Amidation

Huang, Weijie, Taylor, Shelby, Fu, Kefu, Lin, Yi, Zhang, Donghui, Hanks, Timothy W., Rao, Apparao M., Sun, Ya-Ping·2002·Nano Letters

Abstract

Carbon nanotubes are functionalized by bovine serum albumin (BSA) proteins via diimide-activated amidation under ambient conditions. The nanotube-BSA conjugates thus obtained are highly water-soluble, forming dark-colored aqueous solutions. Results from characterizations using atomic force microscopy (AFM), thermal gravimetric analysis, Raman, and gel electrophoresis show that the conjugate samples indeed contain both carbon nanotubes and BSA proteins and that the protein species are intimately associated with the nanotubes. Bioactivities of the nanotube-bound proteins are evaluated using the total protein micro-determination assay (the modified Lowry procedure). The results show that the overwhelming majority (∼90%) of the protein species in the nanotube-BSA conjugates remain bioactive.

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Metadata

Authors
Huang, W., Taylor, S., Fu, K., Lin, Y., Zhang, D., Hanks, T. W., Rao, A. M., & Sun, Y.-P.
Venue
Nano Letters
Published
2002-03-16
Volume
2
Issue
4
Pages
311-314
ISSN
  • 1530-6984 (ISSN-L)
  • 1530-6992
OpenAlex
W1982822058
Cited by
530
References
1
FWCI
19.89
Type
journal article
Language
en
Open access
closed

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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