The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Polymer-Coated NaYF4:Yb3+, Er3+ Upconversion Nanoparticles for Charge-Dependent Cellular Imaging
Abstract
Lanthanide-doped upconversion nanoparticles (UCNPs) are considered promising novel near-infrared (NIR) bioimaging agents with the characteristics of high contrast and high penetration depth. However, the interactions between charged UCNPs and mammalian cells have not been thoroughly studied, and the corresponding intracellular uptake pathways remain unclear. Herein, our research work involved the use of a hydrothermal method to synthesize polyvinylpyrrolidone-coated UCNPs (UCNP-PVP), and then a ligand exchange reaction was performed on UCNP-PVP, with the help of polyethylenimine (PEI) and poly(acrylic acid) (PAA), to generate UCNP-PEI and UCNP-PAA. These polymer-coated UCNPs demonstrated good dispersibility in aqueous medium, had the same elemental composition and crystal phase, shared similar TEM and dynamic light scattering (DLS) size distribution, and exhibited similar upconversion luminescence efficiency. However, the positively charged UCNP-PEI evinced greatly enhanced cellular uptake in comparison with its neutral or negative counterparts, as shown by multiphoton confocal microscopy and inductively coupled plasma mass spectrometry (ICP-MS) measurements. Meanwhile, we found that cationic UCNP-PEI can be effectively internalized mainly through the clathrin endocytic mechanism, as revealed by colocalization, chemical, and genetic inhibitor studies. This study elucidates the role of the surface polymer coatings in governing UCNP-cell interactions, and it is the first report on the endocytic mechanism of positively charged lanthanide-doped UCNPs. Furthermore, this study provides important guidance for the development of UCNPs as specific intracellular nanoprobes, allowing us to control the UCNP-cell interactions by tuning surface properties.
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Cites
The effect of particle design on cellular internalization pathways
Gratton, S. E. A., Ropp, P. A., Pohlhaus, P. D., Luft, J. C., Madden, V. J., Napier, M. E., & DeSimone, J. M. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801763105 · Cited by 2,918
Cited by
Upconversion Nanoparticles: Design, Nanochemistry, and Applications in Theranostics
Chen, G., Qiu, H., Prasad, P. N., & Chen, X. (2014) · Chemical Reviews · doi:10.1021/cr400425h · Cited by 2,699
An overview of nanoparticles commonly used in fluorescent bioimaging
Wolfbeis, O. S. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00392f · Cited by 1,631
The biosafety of lanthanide upconversion nanomaterials
Sun, Y., Feng, W., Yang, P., Huang, C., & Li, F. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00175c · Cited by 309
Li, X., Shen, D., Yang, J., Yao, C., Che, R., Zhang, F., & Zhao, D. (2013) · Chemistry of Materials · doi:10.1021/cm3033498 · Cited by 299
Tian, G., Zhang, X., Gu, Z., & Zhao, Y. (2015) · Advanced Materials · doi:10.1002/adma.201503280 · Cited by 278
Metadata
- Authors
- Jin, J., Gu, Y.-J., Man, C. W.-Y., Cheng, J., Xu, Z., Zhang, Y., Wang, H., Lee, V. H.-Y., Cheng, S. H., & Wong, W.-T.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-09-15
- Volume
- 5
- Issue
- 10
- Pages
- 7838-7847
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2051919656
- Cited by
- 290
- References
- 1
- FWCI
- 12.86
- Type
- journal article
- Language
- en
- Open access
- closed
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