The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Toxicity Mechanisms in Escherichia coli Vary for Silver Nanoparticles and Differ from Ionic Silver
Abstract
Silver nanoparticles (Ag NPs) are commonly added to various consumer products and materials to impair bacterial growth. Recent studies suggested that the primary mechanism of antibacterial action of silver nanoparticles is release of silver ion (Ag(+)) and that particle-specific activity of silver nanoparticles is negligible. Here, we used a genome-wide library of Escherichia coli consisting of ∼4000 single gene deletion mutants to elucidate which physiological pathways are involved in how E. coli responds to different Ag NPs. The nanoparticles studied herein varied in both size and surface charge. AgNO3 was used as a control for soluble silver ions. Within a series of differently sized citrate-coated Ag NPs, smaller size resulted in higher Ag ion dissolution and toxicity. Nanoparticles functionalized with cationic, branched polyethylene imine (BPEI) exhibited equal toxicity with AgNO3. When we used a genome-wide approach to investigate the pathways involved in the response of E. coli to different toxicants, we found that only one of the particles (Ag-cit10) exhibited a pattern of response that was statistically similar to that of silver ion. By contrast, the pathways involved in E. coli response to Ag-BPEI particles were more similar to those observed for another cationic nanoparticle that did not contain Ag. Overall, we found that the pathways involved in bacterial responses to Ag nanoparticles are highly dependent on physicochemical properties of the nanoparticles, particularly the surface characteristics. These results have important implications for the regulation and testing of silver nanoparticles.
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Cites
DAVID: Database for Annotation, Visualization, and Integrated Discovery
Dennis, G., Sherman, B. T., Hosack, D. A., Yang, J., Gao, W., Lane, H. C., & Lempicki, R. A. (2003) · Genome Biology · doi:10.1186/gb-2003-4-5-p3 · Cited by 9,327
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
The bactericidal effect of silver nanoparticles
Morones, J. R., Elechiguerra, J. L., Camacho, A., Holt, K., Kouri, J. B., Ramírez, J. T., & Yacaman, M. J. (2005) · Nanotechnology · doi:10.1088/0957-4484/16/10/059 · Cited by 6,668
Silver nanoparticles as a new generation of antimicrobials
Rai, M., Yadav, A., & Gade, A. (2009) · Biotechnology Advances · doi:10.1016/j.biotechadv.2008.09.002 · Cited by 6,173
Sondi, I., & Salopek-Sondi, B. (2004) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2004.02.012 · Cited by 5,986
Antimicrobial effects of silver nanoparticles
Kim, J. S., Kuk, E., Yu, K. N., Kim, J.-H., Park, S. J., Lee, H. J., Kim, S. H., Park, Y. K., Park, Y. H., Hwang, C.-Y., et al. (2007) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2006.12.001 · Cited by 4,918
Pal, S., Tak, Y. K., & Song, J. M. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02218-06 · Cited by 4,040
Marambio-Jones, C., & Hoek, E. M. V. (2010) · Journal of Nanoparticle Research · doi:10.1007/s11051-010-9900-y · Cited by 2,914
Silver as Antibacterial Agent: Ion, Nanoparticle, and Metal
Chernousova, S., & Epple, M. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201205923 · Cited by 2,264
Negligible Particle-Specific Antibacterial Activity of Silver Nanoparticles
Xiu, Z.-M., Zhang, Q.-B., Puppala, H. L., Colvin, V. L., & Alvarez, P. J. J. (2012) · Nano Letters · doi:10.1021/nl301934w · Cited by 2,108
Strain specificity in antimicrobial activity of silver and copper nanoparticles
Ruparelia, J. P., Chatterjee, A. K., Duttagupta, S. P., & Mukherji, S. (2008) · Acta Biomaterialia · doi:10.1016/j.actbio.2007.11.006 · Cited by 1,885
Jung, W. K., Koo, H. C., Kim, K. W., Shin, S., Kim, S. H., & Park, Y. H. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.02001-07 · Cited by 1,843
Monopoli, M. P., Walczyk, D., Campbell, A., Elia, G., Lynch, I., Baldelli Bombelli, F., & Dawson, K. A. (2011) · Journal of the American Chemical Society · doi:10.1021/ja107583h · Cited by 1,835
Carlson, C., Hussain, S. M., Schrand, A. M., K. Braydich-Stolle, L., Hess, K. L., Jones, R. L., & Schlager, J. J. (2008) · The Journal of Physical Chemistry B · doi:10.1021/jp712087m · Cited by 1,814
Size Dependent and Reactive Oxygen Species Related Nanosilver Toxicity to Nitrifying Bacteria
Choi, O., & Hu, Z. (2008) · Environmental Science & Technology · doi:10.1021/es703238h · Cited by 1,391
Badawy, A. M. E., Luxton, T. P., Silva, R. G., Scheckel, K. G., Suidan, M. T., & Tolaymat, T. M. (2010) · Environmental Science & Technology · doi:10.1021/es902240k · Cited by 1,177
Self-citationKittler, S., Greulich, C., Diendorf, J., Köller, M., & Epple, M. (2010) · Chemistry of Materials · doi:10.1021/cm100023p · Cited by 1,126
Controlled Release of Biologically Active Silver from Nanosilver Surfaces
Liu, J., Sonshine, D. A., Shervani, S., & Hurt, R. H. (2010) · ACS Nano · doi:10.1021/nn102272n · Cited by 1,053
Surface Charge-Dependent Toxicity of Silver Nanoparticles
El Badawy, A. M., Silva, R. G., Morris, B., Scheckel, K. G., Suidan, M. T., & Tolaymat, T. M. (2011) · Environmental Science & Technology · doi:10.1021/es1034188 · Cited by 922
Self-citationLiau, S. Y., Read, D. C., Pugh, W. J., Furr, J. R., & Russell, A. D. (1997) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.1997.00219.x · Cited by 857
120 Years of Nanosilver History: Implications for Policy Makers
Nowack, B., Krug, H. F., & Height, M. (2011) · Environmental Science & Technology · doi:10.1021/es103316q · Cited by 829
Tolaymat, T. M., El Badawy, A. M., Genaidy, A., Scheckel, K. G., Luxton, T. P., & Suidan, M. (2010) · Science of The Total Environment · doi:10.1016/j.scitotenv.2009.11.003 · Cited by 816
Self-citationToxicity of ZnO Nanoparticles to Escherichia coli: Mechanism and the Influence of Medium Components
Li, M., Zhu, L., & Lin, D. (2011) · Environmental Science & Technology · doi:10.1021/es102624t · Cited by 801
Phenotypic Landscape of a Bacterial Cell
Nichols, R. J., Sen, S., Choo, Y. J., Beltrao, P., Zietek, M., Chaba, R., Lee, S., Kazmierczak, K. M., Lee, K. J., Wong, A., et al. (2011) · Cell · doi:10.1016/j.cell.2010.11.052 · Cited by 713
Semple, K. T., Doick, K. J., Jones, K. C., Burauel, P., Craven, A., & Harms, H. (2004) · Environmental Science & Technology · doi:10.1021/es040548w · Cited by 678
Hwang, E. T., Lee, J. H., Chae, Y. J., Kim, Y. S., Kim, B. C., Sang, B., & Gu, M. B. (2008) · Small · doi:10.1002/smll.200700954 · Cited by 468
George, S., Lin, S., Ji, Z., Thomas, C. R., Li, L., Mecklenburg, M., Meng, H., Wang, X., Zhang, H., Xia, T., et al. (2012) · ACS Nano · doi:10.1021/nn204671v · Cited by 366
Self-citationColman, B. P., Arnaout, C. L., Anciaux, S., Gunsch, C. K., Hochella, M. F., Kim, B., Lowry, G. V., McGill, B. M., Reinsch, B. C., Richardson, C. J., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0057189 · Cited by 338
Rosenfeld, Y., & Shai, Y. (2006) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2006.05.017 · Cited by 318
Antimicrobial Activity of Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles
Liong, M., France, B., Bradley, K. A., & Zink, J. I. (2009) · Advanced Materials · doi:10.1002/adma.200802646 · Cited by 262
Self-citationPapo, N., & Shai, Y. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m412865200 · Cited by 253
Stoyanov, J. V., Hobman, J. L., & Brown, N. L. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02264.x · Cited by 241
Gondikas, A. P., Morris, A., Reinsch, B. C., Marinakos, S. M., Lowry, G. V., & Hsu-Kim, H. (2012) · Environmental Science & Technology · doi:10.1021/es3001757 · Cited by 240
Tamae, C., Liu, A., Kim, K., Sitz, D., Hong, J., Becket, E., Bui, A., Solaimani, P., Tran, K. P., Yang, H., et al. (2008) · Journal of Bacteriology · doi:10.1128/jb.01982-07 · Cited by 234
Zook, J. M., Long, S. E., Cleveland, D., Geronimo, C. L. A., & MacCuspie, R. I. (2011) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-011-5266-y · Cited by 233
Biocidal Activity of Nanocrystalline Silver Powders and Particles
Smetana, A. B., Klabunde, K. J., Marchin, G. R., & Sorensen, C. M. (2008) · Langmuir · doi:10.1021/la800091y · Cited by 215
Ivask, A., Rõlova, T., & Kahru, A. (2009) · BMC Biotechnology · doi:10.1186/1472-6750-9-41 · Cited by 201
Self-citationIvask, A., Bondarenko, O., Jepihhina, N., & Kahru, A. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-3962-7 · Cited by 196
Self-citationSilver nanoparticle enhanced silver ion stress response in Escherichia coli K12
McQuillan, J. S., Groenaga Infante, H., Stokes, E., & Shaw, A. M. (2012) · Nanotoxicology · doi:10.3109/17435390.2011.626532 · Cited by 193
Winkelmann, G. (2001) · Wiley eBooks · doi:10.1002/3527600728 · Cited by 120
Klena, J. D., Ashford, R. S., & Schnaitman, C. A. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.22.7297-7307.1992 · Cited by 90
Ralló, R., France, B., Liu, R., Nair, S. S., George, S., Damoiseaux, R. D., Giralt, F., Nel, A. E., Bradley, K. A., & Cohen, Y. (2011) · Environmental Science & Technology · doi:10.1021/es103606x · Cited by 86
Self-citationKim, J. S., Kuk, E., Yu, K. N., Kim, J., Park, S. J., Lee, H.-J., Ys, K., Park, Y. K., Park, Y. H., Hwang, C.-Y., et al. (2014) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2014.04.007 · Cited by 75
Ivask, A., Suarez, E., Patel, T., Boren, D., Ji, Z., Holden, P. A., Telesca, D., Damoiseaux, R. D., Bradley, K. A., & Godwin, H. A. (2011) · Environmental Science & Technology · doi:10.1021/es203087m · Cited by 67
Self-citationQuantitative proteomic profiling of the Escherichia coli response to metallic copper surfaces
Nandakumar, R., Santo, C. E., Madayiputhiya, N., & Grass, G. (2011) · BioMetals · doi:10.1007/s10534-011-9434-5 · Cited by 53
Recruitment of genes and enzymes conferring resistance to the nonnatural toxin bromoacetate
Desai, K. K., & Miller, B. G. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1007559107 · Cited by 32
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Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity
Durán, N., Durán, M., de Jesus, M. B., Seabra, A. B., Fávaro, W. J., & Nakazato, G. (2016) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2015.11.016 · Cited by 1,542
Antibacterial Activity of Silver Nanoparticles: Structural Effects
Tang, S., & Zheng, J. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701503 · Cited by 1,205
Antibacterial properties and toxicity from metallic nanomaterials
Vimbela, G., Ngo, S. M., Fraze, C., Yang, L., & Stout, D. A. (2017) · International Journal of Nanomedicine · doi:10.2147/ijn.s134526 · Cited by 640
Valsami-Jones, E., & Lynch, I. (2015) · Science · doi:10.1126/science.aad0768 · Cited by 232
An evaluation of blood compatibility of silver nanoparticles
Huang, H., Lai, W., Cui, M., Liang, L., Lin, Y., Fang, Q., Liu, Y., & Xie, L. (2016) · Scientific Reports · doi:10.1038/srep25518 · Cited by 186
Metadata
- Authors
- Ivask, A., ElBadawy, A., Kaweeteerawat, C., Boren, D., Fischer, H., Ji, Z., Chang, C. H., Liu, R., Tolaymat, T., Telesca, D., Zink, J. I., Cohen, Y., Holden, P. A., & Godwin, H. A.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-12-24
- Volume
- 8
- Issue
- 1
- Pages
- 374-386
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W1969181076
- Cited by
- 599
- References
- 46
- FWCI
- 13.91
- Type
- journal article
- Language
- en
- Open access
- closed
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