The DOI matches its Crossref record.
Quantifying the Cellular Uptake of Antibody-Conjugated Au Nanocages by Two-Photon Microscopy and Inductively Coupled Plasma Mass Spectrometry
Open accessAbstract
Gold nanocages with localized surface plasmon resonance peaks in the near-infrared region exhibited a broad two-photon photoluminescence band extending from 450 to 650 nm when excited by a Ti:sapphire laser at 800 nm. The bright luminescence makes it possible to explore the use of Au nanocages as a new class of optical imaging agents for two-photon microscopy. In this work, we have demonstrated the use of two-photon microscopy as a convenient tool to directly examine the uptake of antibody-conjugated and PEGylated Au nanocages by U87MGwtEGFR cells. We have also correlated the results from two-photon microscopy with the data obtained by inductively coupled plasma mass spectrometry. Combined together, these results indicate that the antibody-conjugated Au nanocages were attached to the surface of the cells through antibody-antigen binding and then internalized into the cells via receptor-mediated endocytosis. The cellular uptake process was dependent on a number of parameters, including incubation time, incubation temperature, size of the Au nanocages, and the number of antibodies immobilized on each nanocage.
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Citations
Cited by
Engineered Nanoparticles for Drug Delivery in Cancer Therapy
Sun, T., Zhang, Y. S., Pang, B., Hyun, D. C., Yang, M., & Xia, Y. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201403036 · Cited by 2,164
Gold Nanomaterials at Work in Biomedicine
Yang, X., Yang, M., Pang, B., Vara, M., & Xia, Y. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00193 · Cited by 1,162
Self-citationSoenen, S. J., Rivera-Gil, P., Montenegro, J.-M., Parak, W. J., De Smedt, S. C., & Braeckmans, K. (2011) · Nano Today · doi:10.1016/j.nantod.2011.08.001 · Cited by 699
Metadata
- Authors
- Au, L., Zhang, Q., Cobley, C. M., Gidding, M., Schwartz, A. G., Chen, J., & Xia, Y.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-12-02
- Volume
- 4
- Issue
- 1
- Pages
- 35-42
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2010673136
- Cited by
- 162
- References
- 0
- FWCI
- 6.15
- Type
- journal article
- Language
- en
- Open access
- green
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