A Direct Bioautographic Tlc Assay for Compounds Possessing Antibacterial Activity
Abstract
A simple bioassay for the direct detection of antibacterial compounds on tlc plates has been developed. A series of natural products and different stationary phases were tested in order to establish the utility of the assay for the isolation of antibacterial compounds from higher plants.
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For bacteria, Bacillus subtilis, Staphylococcus aureus and Escherichia coli strains are frequently used to identify antibacterial compounds in direct bioautography.
“For bacteria, Bacillus subtilis, Staphylococcus aureus and Escherichia coli strains are frequently used to identify antibacterial compounds [42], [53].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [53]
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Methods for in vitro evaluating antimicrobial activity: A review
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2015.11.005 · Cited by 7,264 · see citing works
- Authors
- Hamburger, M. O., & Cordell, G. A.
- Venue
- Journal of Natural Products
- Publisher
- American Chemical Society (ACS)
- Published
- 1987-01
- Volume
- 50
- Issue
- 1
- Pages
- 19-22
- ISSN
- 0163-3864 (ISSN-L)
- 1520-6025
- OpenAlex
- W2095415622
- Cited by
- 205
- References
- 0
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 4 · 2022: 1 · 2021: 8 · 2020: 7 · 2019: 6 · 2018: 9 · 2017: 9 · 2016: 4 · 2015: 8 · 2014: 11 · 2013: 13 · 2012: 6
- FWCI
- 2.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Natural Products (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- National Cancer Institute (CA 20164)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive natural compounds
- Keywords
- antibacterial agents, bioautography, natural products, higher plants
- MeSH terms
- Anti-Bacterial Agents, Bacillus subtilis, Chromatography, Thin Layer, Culture Media, Escherichia coli
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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