The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Relationship between cell size, cell cycle and specific recombinant protein productivity
Open accessAbstract
Centrifugal elutriation was used to produce cell cycle enrichedfractions of four commercially relevant recombinant cell lines,chosen to allow for variation in properties due to construct,expression system and parent cell type, from normally growingheterogeneous batch cultures. As these fractions had identicalculture histories and had not been subjected to any insult orstress which was likely to have adversely affected cellularmetabolism, they were ideal for further study of cellularproperties. Specific productivity, cell size and cell cyclestate of replicate elutriated fractions were measured for eachcell line. Results showed that cell size was the major cellulardeterminant of productivity for all cell lines examined. Productformation was not restricted to any particular cell cycle phaseand in all cases, production occurred irrespective of cell cyclephase. Specific productivity was lowest when the majority ofcells in the fraction were G(1), intermediate when themajority of cells in the fraction were S phase and greater whenthe majority of cells in the fraction were in G(2)/M. However, the evidence suggests that size is the major cellulardeterminant of productivity; the apparent relationship betweencell cycle and productivity is secondary and can simply beascribed to the increasing size of cells as they progress thoughthe cell cycle. Thus, in addition to cell density and viabilitycell size is the cellular parameter which should be incorporatednot only into mathematical models of recombinant mammalian cellproduction processes but also into process monitoring andcontrol strategies.
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“This observation was in concordance with other studies that suggested that the increase in cell size was the major determinant of high productivity.[14,22,23]”
From Biomass specific perfusion rate as a control lever for the continuous manufacturing of biosimilar monoclonal antibodies from CHO cell cultures (2024) · cites this paper as [23]
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Cites
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Self-citationExpression in COS cells of a mouse—human chimaeric B72.3 antibody
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Research works printed but not linked: 1
- Lloyd DR and Al-Rubeai M (1999) Cell cycle. In: Flickinger MC and Drew SW (eds) Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation (pp. 465–476) John Wiley and Sons, New York.
Cited by
Intraclonal Protein Expression Heterogeneity in Recombinant CHO Cells
Pilbrough, W., Munro, T. P., & Gray, P. P. (2009) · PLoS ONE · doi:10.1371/journal.pone.0008432 · Cited by 210
Imaging Flow Cytometry: Coping with Heterogeneity in Biological Systems
Barteneva, N. S., Fasler‐Kan, E., & Vorobjev, I. A. (2012) · Journal of Histochemistry & Cytochemistry · doi:10.1369/0022155412453052 · Cited by 193
Guidelines to cell engineering for monoclonal antibody production
Rita Costa, A., Elisa Rodrigues, M., Henriques, M., Azeredo, J., & Oliveira, R. (2010) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2009.10.002 · Cited by 178
Dinnis, D. M., & James, D. C. (2005) · Biotechnology and Bioengineering · doi:10.1002/bit.20499 · Cited by 162
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Metadata
- Authors
- Lloyd, D. R., Holmes, P., Jackson, L. P., Emery, A. N., & Al-Rubeai, M.
- Affiliations
- University of Birmingham (GB)
- ORCID
- Lloyd, David R.
- Venue
- Cytotechnology
- Published
- 2000-10
- Volume
- 34
- Issue
- 1-2
- Pages
- 59-70
- ISSN
- 0920-9069 (ISSN-L)
- 1573-0778
- PMC
- PMC3449736
- OpenAlex
- W1554602439
- Cited by
- 93
- References
- 31
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 5 · 2019: 5 · 2018: 7 · 2017: 2 · 2016: 6 · 2015: 1 · 2014: 6 · 2013: 3 · 2012: 2
- FWCI
- 0.36
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cytotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- cell size, cell cycle, centrifugal elutriation, recombinant cell lines, G1 phase, S phase, G2/M phase, batch culture, process monitoring, process control
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- Lloyd, D. R., Holmes, P., Jackson, L. P., Emery, A. N., & Al-Rubeai, M. (2000). Relationship between cell size, cell cycle and specific recombinant protein productivity. Cytotechnology. https://doi.org/10.1023/a%3A1008103730027
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
