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Cited by 24Cellular transport and secretionAutophagy in Disease and TherapyCalcium signaling and nucleotide metabolism

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A Role for Small GTPase RhoA in Regulating Intracellular Membrane Traffic of Lysosomes in Invasive Rat Hepatoma Cells

Nishimura, Yukio, Itoh, Kazuyuki, Yoshioka, Kiyoko, Ikeda, Kazuhiko, Himeno, Masaru·2002·The Histochemical Journal
DOI 10.1023/a:1021702812146
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Abstract

Small GTPase RhoA regulates signal transduction from receptors in the membrane to a variety of cellular events related to cell morphology, motility, cytoskeletal dynamics, cytokinesis, and tumour progression, but it is unclear how RhoA regulates intracellular membrane dynamics of lysosomes. We showed previously by confocal immunofluorescence microscopy that the transfection of dominant active RhoA in MM1 cells causes the dispersal translocation of lysosomes stained for cathepsin D throughout the cytoplasm. Y-27632, a selective inhibitor of p160ROCK, impeded the cellular redistribution of lysosomes and promoted reclustering of lysosomes toward the perinuclear region. Here we have further investigated whether the acidic lysosomal vesicles dispersed throughout the cytoplasm are applied to the early endosomes in the endocytic pathway, and we demonstrate that the dispersed lysosomes were accessible to endocytosed molecule such as dextran, and their acidity was not changed, as determined by increased accumulation of the acidotropic probe LysoTracker Red. Brefeldin A did not induce the tabulation of these dispersed lysosomes, but it caused early endosomes to form an extensive tubular network. The dispersed lysosomes associated with cathepsin D and LIMPII were not colocalized with early endosomes, and these vesicles were not inaccessible to the endocytosed anti-transferrin receptor antibody. Moreover, wortmannin, an inhibitor of phosphatidylinositol 3-kinase, induced a dramatic chang…

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Research works printed but not linked: 51

  1. Amano M, Chihara K, Kimura Y, Fukata N, Nakamura Y, Matsuura Y, Kaibuchi K (1997) Formation of actin stress fibers and focal adhesions enhanced by Rho-kinase. Science 275: 1308-1311. · DOI · Paper page
  2. Aniento F, Emans N, Griffiths G, Gruenberg J (1993) Cytoplasmic dynein-dependent vesicular transport from early to late endosomes. J Cell Biol 123: 1373-1387. · DOI
  3. Brown WJ, Emr SD, Plutner H, Balch WE (1995) Role for phosphatidylinositol 3-kinase in the sorting and transport of newly synthesized lysosomal enzymes in mammalian cells. J Cell Biol 130: 797-805. · DOI
  4. Bucci C, Thomsen P, Nicoziani P, McCarthy J, van Deurs B (2000) Rab7: A key to lysosome biogenesis. Mol Biol Cell 11: 467-480. · DOI
  5. Chavrier P, Goud B (1999) The role of ARF and Rab GTPases in membrane transport. Curr Opin Cell Biol 11: 466-475. · DOI
  6. Cook TA, Nagasaki T, Gundersen GG (1998) Rho guanosine triphosphatase mediates the selective stabilization of microtubules induced by lysophosphatidic acid. J Cell Biol 141: 175-185. · DOI
  7. Davidson H (1995) Wortmannin causes mis-targeting of procathepsin D. Evidence for the involvement of a phosphatidylinositol 3-kinase in vesicular transport to lysosomes. J Cell Biol 130: 797-805. · DOI
  8. Griffiths G, Hoflack B, Simons K, Mellman I, Kornfeld S (1988) The mannose 6-phosphate receptor and the biogenesis of lysosomes. Cell 52: 329-341. · DOI
  9. Hall A (1994) Small GTP-binding proteins and the regulation of the actin cytoskeleton. Annu Rev Cell Biol 10: 31-54. · DOI
  10. Hall A (1998) Rho GTPases and the actin cytoskelton. Science (Washington DC) 279: 509-514. · DOI
  11. Hirose M, Ishizaki T, Watanabe N, Uehata M, Kranenburg O, Moolenaar WH, Matsumura F, Maekawa M, Bito H, Narumiya S (1998) Molecular dissection of the Rho-associated kinase (p160ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells. J Cell Biol 141: 1625-1636. · DOI
  12. Hollenbeck PJ, Swanson JA (1990) Radial extension of macrophage tubular lysosomes supported by kinesin. Nature 346: 864-866. · DOI
  13. Ishizaki T, Naito M, Fujisawa K, Maekawa M, Watanabe, N, Saito Y, Narumiya S (1997) p160ROCK, a Rho-associated coiled-coil forming protein kinase, works downstream of Rho and induces focal adhesion. FEBS Lett 404: 118-124. · DOI
  14. Itoh K, Yoshioka K, Akedo H, Uehata M, Ishizaki T, Narumiya S (1999) Anessential part for Rho-associated kinase in the transcellular invasion of tumor cells. Nature Medicine 5: 221-225. · DOI
  15. Kageshita T, Yoshii A, Kimura T, Maruo K, Ono T, Himeno M, Nishimura Y (1995) Biochemical and immunohistochemical analysis of cathepsins B, H, L and D in human melanocytic tumours. Arch Dermatol Res 287: 266-272. · DOI
  16. Kishi K, Sasaki T, Kuroda S, Itoh T, Takai Y (1993) Regulation of cytoplasmic division of Xenopus Embryo by rhop21 and its inhibitory GDP/GTP exchange protein (rhoGDI). J Cell Biol 120: 1187-1195. · DOI
  17. Kornfeld S, Mellman I (1989) The biogenesis of lysosomes. Ann Rev Cell Biol 5: 483-525. · DOI
  18. Leung T, Manser E, Tan L, Lim L (1995) A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to the peripheral membranes. J Biol Chem 270: 29051-29054. · DOI
  19. Lin SXH, Collins CA (1992) Immunolocalization of cytoplasmic dynein to lysosomes in cultured cells. J Cell Sci 101: 125-137. · DOI
  20. Lippincott-Schwartz J, Fambrough DM (1986) Lysosomal membrane dynamics: Structure and interorganellar movement of a major lysosomal membrane glycoprotein. J Cell Biol 102: 1593-1605. · DOI
  21. Lippincott-Schwartz J, Yuan L, Tripper C, Amherdt M, Orci L, Klausner RD (1991) Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67: 601-616. · DOI
  22. Matsui T, Amano M, Yamamoto T, Chihara K, Nakafuku M, Ito M, Nakano T, Okawa K, Iwamatsu A, Kaibuchi K (1996) Rho-associated kinase, a novel serine threonine kinase, as a putative target for the small GTP binding protein Rho. EMBO J 15: 2208-2216. · DOI
  23. Matteoni R, Kreis TE (1987) Translocation and clustering of endosomes and lysosomes depends on microtubules. J Cell Biol 105: 1253-1265. · DOI
  24. Nakagawa O, Fujisawa K, Ishizaki T, Saito Y, Nakao K, Narumiya S (1996) ROCK-I and ROCK-II; two isoforms of Rho-associated coiledcoil forming protein serine/threonine kinase in mice. FEBS Lett 392: 189-193. · DOI
  25. Nishimura Y, Higaki M, Kato K (1987) Identification of a precursor form of cathepsin D in microsomal lumen: Characterization of enzymatic activation and proteolytic processing in vitro. Biochem Biophys Res Commun 148: 335-343. · DOI
  26. Nishimura Y, Kawabata T, Kato K (1988) Identification of latent procathepsins B and L in microsomal lumen: Characterization of enzymatic activation and proteolytic processing iin vitro. Arch Biochem Biophys 261: 64-71. · DOI
  27. Nishimura Y, Sameni M, Sloane BF (1998) Malignant transformation alters intracellular trafficking of lysosomal cathepsinDin human breast epithelial cells. Pathol Oncol Res 4: 283-296. · DOI
  28. Nishimura Y, Itoh K, Yoshioka K, Uehata M, Himeno M (2000) Small guanosine triphosphatase Rho/Rho-associated kinase as a novel regulator of intracellular redistribution of lysosomes in invasive tumor cells. Cell Tissue Res 342: 341-351. · DOI · Paper page
  29. Okazaki I, Himeno M, Ezaki J, Ishikawa T, Kato K (1992) Purification and characterization of an 85 kDa sialoglycoprotein in rat liver. J Biochem 111: 763-769. · DOI
  30. Paterson HF, Self AJ, Garrett MD, Just I, Aktories K, Hall A (1990) Microinjection of recombinant p21rho induces rapid changes in cell morphorogy. J Cell Biol 111: 1001-1007. · DOI
  31. Perona R, Esteve P, Jimenez B, Ballestero RP, Cajal SR, LacaL JC (1993) Tumorigenic activity of rho genes from Aphysia California. Oncogene 8: 1285-1292.
  32. Pfeffer SR (1992) GTP-binding proteins in intracellular transport. Trends Cell Biol 2: 41-46. · DOI
  33. Pfeffer SR (1999) Transport-vesicle targeting: Tethers before SNAREs. Nat Cell Biol 1: E17-E22. · DOI
  34. Prendergast GC, Khosravi-Far R, Solski PA, Kurzawa H, Lebowitz PF, Der CJ (1995) Critical role of Rho in cell transformation by oncogenic Ras. Oncogene 10: 2289-2296.
  35. Press B, Feng Y, Hoflack B, Wandinger-Nes A (1998) Mutant Rab7 causes the accumulation of cathepsin D and cation-independent mannose 6-phosphate receptor in an early endocytic compartment. J Cell Biol 140: 1075-1089. · DOI
  36. Reaves B, Bright NA, Mullock B, Luzio JP (1996) The effect of wortmannin on the localization of lysosomal type I integral membrane glycoproteins suggests a role for phosphoinositide 3-kinase activity in regulating membrane traffic late in the endocytic pathway. J Cell Sci 109: 749-762. · DOI
  37. Rochefort H, Capony F, Garcia M (1990) Cathepsin D: A protease involved in cancer metastasis. Cancer Metast Rev 9: 321-331. · DOI
  38. Sameni M, Elliott E, Ziegler G, Fortgens PH, Dennison C, Sloane BF (1995) Cathepsin B and D are localized at the surface of human breast cancer cells. Pathol Oncol Res 1: 43-53. · DOI
  39. Sandoval IV, Arredondo JJ, Alcalde J, Gonzalez-Noriega A, Vandekerckhove J, Jimenez MA, Rico M (1994) The residues Leu(Ile)475-Ile(Leu)476, contained in the extended carboxyl cytoplasmic tail, are critical for targeting of the resident lysosomal membrane protein LIMPII to lysosomes. J Biol Chem 269: 6622-6631. · DOI
  40. Shpetner H, Joly M, Hartley D, Corvera S (1996) Potential sites of PI 3-kinase function in the endocytic pathway revealed by the PI 3-kinase inhibitor, wortmannin. J Cell Biol 132: 595-605. · DOI
  41. Sloane BF, Moin K, Sameni M, Tait LR, Rozhin J, Ziegler G (1994) Membrane association of cathepsin B can be induced by transfection of human breast epithelial cells with c-Ha-ras oncogene. J Cell Sci 107: 373-384. · DOI
  42. Stack JH, Emr SD (1993) Genetic and biochemical studies of protein sorting to the yeast vacuole. Curr Biol 5: 641-646. · DOI
  43. Tabuchi N, Akasaki K, Tsuji H (2000) Two acidic amino acid residues, Asp(470) and Glu(471), contained in the carboxyl cytoplasmic tail of a major lysosomal membrane protein, LGP85/LIMPII, are important for its accumulation in secondary lysosomes. Biochem Biophys Res Commun 270: 557-563. · DOI
  44. Takaishi K, Kikuchi A, Kuroda S, Kotani K, Sasaki T, Takai Y (1993) Involvement of rhop21 and its inhibitory GDP/GTP exchange protein (rhoGDI) in cell motility. Mol Cell Biol 13: 72-79.
  45. Taniguchi S, Nishimura Y, Takahashi T, Baba T, Kato K (1990) Augmented excretion of procathepsin L of a fos-transferred highly metastatic cell line. Biochem Biophys Res Commun 168: 520-526. · DOI
  46. Timar J, Tang D, Bazaz R, Kimler V, Taylor JD, Honn KV (1993) PKC mediates 12-(S)-HETE-induced cytoskeletal rearrangement in B16a melanoma cells. Cell Motil Cytoskell 26: 49-65. · DOI
  47. Timar J, Bazaz R, Kimler V, Haddad M, Tang DG, Robertson D, Tovari J, Taylor JD, Honn KV (1995) Immunomorphological characterization and effects of 12-(S)-HETE on a dynamic intracellular pool of the ?IIb?3-integrin in melanoma cells. J Cell Sci 108: 2175-2186. · DOI
  48. Uehata M, Ishizaki T, Satoh H, Ono T, Kawahara T, Morishita T, Tamakawa H, Yamagami K, Inui J, Maekawa M, Narumiya S (1997) A key role for p160ROCK-mediated Ca++ sensitization of smooth muscle in hypertension. Nature 389: 990-994. · DOI
  49. Yoshioka K, Matsumura F, Akedo H, Itoh K (1998) Small GTP-binding protein Rho stimulates the actomyosin system, leading to invasion of tumor cells. J Biol Chem 273: 5146-5154. · DOI
  50. Yoshioka K, Nakamori S, Itoh K (1999) Overexpression of small GTPbinding protein RhoA promotes invasion of tumor cells. Cancer Res 59: 2004-2010.
  51. Zerial M, Stenmark H (1993) Rab GTPases in vesicular transport. Curr Opin Cell Biol 5: 613-620. · DOI

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Metadata

Authors
Nishimura, Y., Itoh, K., Yoshioka, K., Ikeda, K., & Himeno, M.
Affiliations
  • Kyushu University (JP)
  • Osaka International Cancer Institute (JP)
ORCID
Venue
The Histochemical Journal
Publisher
Springer Science and Business Media LLC
Published
2002-05
Volume
34
Issue
5
Pages
189-213
ISSN
  • 0018-2214 (ISSN-L)
  • 1573-6865
DOI
10.1023/a:1021702812146
OpenAlex
W1540525572
Cited by
24
References
0
Citations by year
2025: 1 · 2022: 2 · 2020: 3 · 2017: 1 · 2016: 1 · 2014: 2 · 2012: 1
FWCI
0.68
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • The Histochemical Journal (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
RhoA, hepatoma cells, cathepsin D, early endosomes, endocytic pathway, Y-27632, wortmannin, phosphatidylinositol 3-kinase, transferrin receptor, brefeldin A, membrane tubulation
MeSH terms
Wortmannin, Androstadienes, Dextrans, Endocytosis, Enzyme Inhibitors, Carcinoma, Hepatocellular, Intracellular Membranes, Lysosomes, Microscopy, Fluorescence, Receptors, Transferrin, Endosomes, Transfection, Tumor Cells, Cultured, Signal Transduction, Microscopy, Confocal, CD36 Antigens, Brefeldin A, rho GTP-Binding Proteins

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CoChat. (2026). Citation check: A Role for Small GTPase RhoA in Regulating Intracellular Membrane Traffic of Lysosomes in Invasive Rat Hepatoma Cells. https://app.cochat.ai/papers/10.1023/a:1021702812146
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Nishimura, Y., Itoh, K., Yoshioka, K., Ikeda, K., & Himeno, M. (2002). A Role for Small GTPase RhoA in Regulating Intracellular Membrane Traffic of Lysosomes in Invasive Rat Hepatoma Cells. The Histochemical Journal. https://doi.org/10.1023/a%3A1021702812146