Intracellular Visualization of Ampicillin-Loaded Nanoparticles in Peritoneal Macrophages Infected in Vitro with Salmonella typhimurium
Abstract
Intracellular targeting of ampicillin by means of polyisohexylcyanoacrylate (PIHCA) nanoparticles was studied in murine peritoneal macrophages infected with Salmonella typhimurium. The intracellular distribution of actively endocytosed nanoparticles was visualized by transmission electron microscopy and confocal microscopy. Nanoparticles were either isolated or closely associated with bacteria within phagosomes or phagolysosomes. Thus the potential of ampicillin-loaded nanoparticles in targeting of intracellular bacteria is demonstrated. Consequently, ampicillin, which usually penetrates into cells at a low level, is directly carried in, when loaded on nanoparticles, and brought into contact with intracellular bacteria.
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Poly(cyanoacrylate) nanoparticles colocalized with S. typhirium in phagosomes of macrophages, resulting in enhanced intracellular targeting of ampicillin.
“Couvreur and colleagues showed that poly(cyanoacrylate) nanoparticles colocalized with S. typhirium in phagosomes of macrophages, resulting in enhanced intracellular targeting of ampicillin 79 .”
From Nanotechnology approaches for global infectious diseases (2021) · cites this paper as [79]
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References 25 · Cited by 53
- Authors
- Pinto-Alphandary, H., Balland, O., Laurent, M., Andremont, A., Puisieux, F., & Couvreur, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Université Paris-Sud (FR)
- Institut Gustave Roussy (FR)
- Venue
- Pharmaceutical Research
- Published
- 1994-01
- Volume
- 11
- Issue
- 1
- Pages
- 38-46
- ISSN
- 0724-8741 (ISSN-L)
- 1573-904X
- OpenAlex
- W338173593
- Cited by
- 53
- References
- 25
- Citations by year
- 2024: 3 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 5
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Pharmaceutical Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- peritoneal macrophages, Salmonella typhimurium, intracellular drug delivery, phagosome, phagolysosomes, bacterial infection, nanoparticle endocytosis, subcellular targeting
- MeSH terms
- Ampicillin, Animals, Cyanoacrylates, Endocytosis, Mice, Inbred Strains, Microscopy, Electron, Microspheres, Phagosomes, Salmonella Infections, Animal, Salmonella typhimurium, Macrophages, Peritoneal, Mice, In Vitro Techniques
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