The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Induction of Autophagy Causes Dramatic Changes in the Subcellular Distribution of GFP‐Rab24
Open accessAbstract
Rab GTPases comprises a large family of proteins, with more than 50 gene products localized in distinct subcellular compartments. Rab24 is a member of this family whose function is not presently known. In order to elucidate the role of this protein we have generated a GFP‐tagged Rab24 and studied the distribution of this chimera by fluorescence microscopy. GFP‐Rab24 showed a perinuclear reticular localization that often encircled the nucleus. This reticular pattern partially overlapped with ER markers, cis‐Golgi, and the ER‐Golgi intermediate compartment. Surprisingly, when GFP‐Rab24‐transfected cells were starved to induce autophagy the distribution of the protein changed dramatically. GFP‐Rab24 localized in large dots, cup‐shaped structures and ring‐shaped vesicles. Some of these vesicles were labeled with monodansylcadaverine , a specific autophagosome marker. In the presence of vinblastine, an agent that induces the formation of very large autophagic vesicles, GFP‐Rab24 accumulated in the large vacuoles that were also labeled by monodansylcadaverine. Furthermore, Rab24 colocalized with LC3, a mammalian homolog of the yeast protein Apg8/Aut7, an essential gene for autophagy. This is the first report indicating that Rab24 localizes on autophagosomes, suggesting that this Rab protein is involved in the autophagic pathway.
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Cites
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y., & Yoshimori, T. (2000) · The EMBO Journal · doi:10.1093/emboj/19.21.5720 · Cited by 6,605
Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
Kirisako, T., Baba, M., Ishihara, N., Miyazawa, K., Ohsumi, M., Yoshimori, T., Noda, T., & Ohsumi, Y. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.2.435 · Cited by 881
Isolation and Characterization of Rat Liver Amphisomes
Berg, T. O., Fengsrud, M., Strømhaug, P. E., Berg, T., & Seglen, P. O. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.34.21883 · Cited by 385
Prelysosomal convergence of autophagic and endocytic pathways
Gordon, P. B., & Seglen, P. O. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(88)90556-6 · Cited by 281 · cited 1×
“Vinblastine, an agent that disrupts microtubules, prevents the maturation of autophagosomes by blocking fusion with lysosomes (22,23).”
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Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationExtensive Involvement of Autophagy in Alzheimer Disease: An Immuno-Electron Microscopy Study
Nixon, R. A., Wegiel, J., Kumar, A., Yu, W. H., Peterhoff, C., Cataldo, A., & Cuervo, A. M. (2005) · Journal of Neuropathology & Experimental Neurology · doi:10.1093/jnen/64.2.113 · Cited by 1,588
Macroautophagy—a novel β-amyloid peptide-generating pathway activated in Alzheimer's disease
Yu, W. H., Cuervo, A. M., Kumar, A., Peterhoff, C. M., Schmidt, S. D., Lee, J.-H., Mohan, P. S., Mercken, M., Farmery, M. R., Tjernberg, L. O., et al. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200505082 · Cited by 967
Pankiv, S., Alemu, E. A., Brech, A., Bruun, J.-A., Lamark, T., Øvervatn, A., Bjørkøy, G., & Johansen, T. (2010) · Journal of Cell Biology · doi:10.1083/jcb.200907015 · Cited by 659
Metadata
- Authors
- Munafó, D. B., & Colombo, M. I.
- Venue
- Traffic
- Publisher
- Wiley
- Published
- 2002-06-05
- Volume
- 3
- Issue
- 7
- Pages
- 472-482
- ISSN
- 1398-9219 (ISSN-L)
- 1600-0854
- OpenAlex
- W2034766627
- Cited by
- 182
- References
- 4
- FWCI
- 7.61
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Munafó, D. B., & Colombo, M. I. (2002). Induction of Autophagy Causes Dramatic Changes in the Subcellular Distribution of GFP‐Rab24. Traffic. https://doi.org/10.1034/j.1600-0854.2002.30704.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
