The DOI matches its Crossref record.
Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo
Abstract
The widespread use of clomiphene citrate and exogenous gonadotrophins for in vitro fertilization (IVF) in human frequently results in the production of multiple embryos. Replacement of more than two embryos increases pregnancy rate but may result in multiple pregnancies with increased pre- and post-natal abnormality. Preservation of embryos for a limited time allows fewer embryos to be replaced on several different occasions and thus the problems of multiple pregnancy can be minimized, the effectiveness of a single IVF procedure increased and embryo replacement in adverse maternal conditions avoided. Preimplantation embryos have been successfully cryopreserved in many animal species. The sensitivity of embryos to cooling and freezing varies between species and stages of embryo development. We report here the cryopreservation procedures that allow a high survival rate of four- and eight-cell human embryos and the establishment of a pregnancy following the freezing and storage of an eight-cell embryo for 4 months in liquid nitrogen. The pregnancy terminated at 24 weeks' gestation due to development of a septic Streptomyces agalactiae chorion amnionitis after premature membrane rupture.
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Use of frozen embryo transfer in in-vitro fertilisation has increased.
“Use of frozen embryo transfer (FET) in in-vitro fertilisation (IVF) has increased.”
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Research works printed but not linked: 14
- Trounson, A. O., Leeton, J. F., Wood, C., Webb, J. & Wood, J. Science 212, 681–682 (1981). · DOI
- Trounson, A. O. & Conti, A. Br. med. J. 285, 244–248 (1982). · DOI
- Trounson, A. O., Mohr, L. R., Wood, C. & Leeton, J. F. J. Reprod. Fert. 64, 285–294 (1982). · DOI
- Trounson, A. O., Shea, B. F., Ollis, G. W. & Jacobson, M. E. J. anim. Sci. 47, 667–681 (1978). · DOI
- Mohr, L. R. & Trounson, A. O. Biol. Reprod. 25, 1009–1025 (1981). · DOI
- Whittingham, D. G. J. Reprod. Fert. 49, 89–94 (1977). · DOI
- Mohr, L. R. thesis, Monash Univ. (1983).
- Willadsen, S. M. Nature 277, 298–300 (1979). · DOI
- Willadsen, S. M. J. Embryol. exp. Morph. 65, 165–172 (1981).
- Trounson, A. O., Wood, C. & Leeton, J. F. Med. J. Aust. 2, 332–334 (1982). · DOI
- Whittingham, D. G. Nature 233, 125–126 (1971). · DOI
- Trounson, A. O. in Current Topics in Experimental Endocrinology Vol. 5, (eds Martini, V. & James, J.) 44–73 (Academic, London, 1983).
- Wood, C., Leeton, J. F., Talbot, J. Mc. & Trounson, A. O. Br. J. Obstet. Gynaecol. 88, 756–760 (1981). · DOI
- Leeton, J., Trounson, A. O., Jessup, D. & Wood, C. Fert. Steril. 38, 156–161 (1982). · DOI
Cited by
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Ho, V. N. A., Pham, T. D., Nguyen, N. T., Wang, R., Norman, R. J., Mol, B. W., Ho, T. M., & Vuong, L. N. (2024) · The Lancet · doi:10.1016/s0140-6736(24)00756-6 · Cited by 55
Metadata
- Authors
- Trounson, A., & Mohr, L.
- Affiliations
- Monash University (AU)
- ORCID
- Trounson, Alan
- Venue
- Nature
- Published
- 1983-10
- Volume
- 305
- Issue
- 5936
- Pages
- 707-709
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2031323095
- Cited by
- 1,126
- References
- 0
- Citations by year
- 2026: 20 · 2025: 21 · 2024: 27 · 2023: 40 · 2022: 42 · 2021: 45 · 2020: 46 · 2019: 49 · 2018: 35 · 2017: 29 · 2016: 31 · 2015: 32 · 2014: 39 · 2013: 39 · 2012: 44
- FWCI
- 37.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- DigitalGeorgetown (Georgetown University Library) (submittedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Assisted Reproductive Technology and Twin Pregnancy
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
- Keywords
- embryo cryopreservation, human embryo, eight-cell embryo, embryo transfer, in vitro fertilization, multiple pregnancy, embryo survival, liquid nitrogen storage, Streptococcus agalactiae, amnionitis, premature membrane rupture
- MeSH terms
- Bacterial Infections, Dimethyl Sulfoxide, Embryo Transfer, Female, Fertilization in Vitro, Fetal Death, Fetal Membranes, Premature Rupture, Premature Rupture of Fetal Membranes, Freezing, Humans, Pregnancy, Streptomyces, Tissue Preservation, Risk Assessment, Parental Consent
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