A mouse locus at which transcription from both DNA strands produces mRNAs complementary at their 3′ ends
Abstract
The organization and large size of the mammalian cell genome allows spatial separation of different transcription units. In those cases where more than one species of messenger are synthesized from the same cellular DNA sequence, they have been found to be generated from transcription proceeding in the same direction. These mRNAs always share regions of homology and can differ from one another as a result of differential processing (splicing and/or polyadenylation) or alternative initiation. In contrast, complementary mRNAs transcribed from opposite strands of the same cellular DNA sequence have not previously been observed. Here we have identified a region of mouse DNA at which processed mRNAs from two adjacent convergent transcription units overlap by 133 base pairs (bp) at their 3'-untranslated ends. One of the transcription units appears to encode a second mRNA which does not contain this overlapping region. This represents the first description of the natural occurrence of processed mammalian cell mRNAs transcribed from opposite strands of the same DNA sequence. The implications of these complementary regions in normal gene regulation are discussed in the context of the finding that the artificial introduction into cells of DNA constructs synthesizing anti-sense RNAs complementary to regions of mRNA transcribed from a chromosomal gene, can inhibit the gene's activity, presumably by the formation of double-stranded RNA.
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NATs have become significant regulators of gene expression.
“Once considered transcriptional noise, also similar to all of the non-coding RNAs, as the case of NATs’ weight of evidence has fast evolved throughout the years, they have become a significant regulator of gene expression.”
From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [63]
NATs are capable of being active in all stages of the transfer of information in the central dogma for transcription, translation, replication, and RNA degradation.
“NATs are capable of being active in all stages of the transfer of information in the central dogma for transcription, translation, replication, sometimes conventionally named differently, and also RNA degradation.”
From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [63]
NATs display conservation across the kingdoms of life and are involved in transcription or in ncRNA genesis.
“They display conservation all across the kingdoms of life, being involved in transcription or in ncRNA genesis.”
From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [63]
NATs can contribute to gene expression switching transcription and can act within protein complexes as a modular scaffold; in both functions they can rewire expression machinery.
“Either way, it could contribute to gene expression switching transcription, and also, it can act within protein/complexes as a modular scaffold; in both functions, it can rewire expression machinery [63].”
From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [63]
NATs' genomic structure enables regulation of their expression in cis, and they could also regulate expression in transposition depending on domain size.
“Their genomic structure enables the regulation of their expression in cis, but could also regulate expression in transposition, although it depends on how large the domain is.”
From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [63]
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References 50 · Cited by 184
- Authors
- Williams, T., & Fried, M.
- ORCID
- Williams, Trevor
- Venue
- Nature
- Published
- 1986-07
- Volume
- 322
- Issue
- 6076
- Pages
- 275-279
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2043981870
- Cited by
- 184
- References
- 50
- Citations by year
- 2024: 3 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 4 · 2014: 1 · 2013: 2
- FWCI
- 6.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- convergent transcription, antisense mRNA, gene expression regulation, antisense RNA, double-stranded RNA, polyadenylation, splicing, differential processing
- MeSH terms
- Animals, Base Composition, DNA, Gene Expression Regulation, Genes, Regulator, Mice, Inbred BALB C, Nucleic Acid Conformation, Poly A, RNA, Double-Stranded, RNA, Messenger, Sequence Homology, Nucleic Acid, Transcription, Genetic, Mice
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