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Mycoplasma infection of cultured cells
Abstract
Mycoplasma contamination is tough to detect and even more difficult to eradicate. It is best to start over fresh from clean cell stocks, but several elimination options are available.
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“Mycoplasma contamination, in particular, can persist undetected in cell cultures for extended periods, resulting in substantial alterations in gene expression and cellular behavior.”
From Cell Immortality: In Vitro Effective Techniques to Achieve and Investigate Its Applications and Challenges (2024) · cites this paper as [161]
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Research works printed but not linked: 14
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Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR
van Kuppeveld, F. J., Johansson, K. E., Galama, J. M., Kissing, J., Bölske, G., van der Logt, J. T., & Melchers, W. J. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.1.149-152.1994 · Cited by 186
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Amyloid-beta peptide degradation in cell cultures by mycoplasma contaminants
Zhao, H., Dreses-Werringloer, U., Davies, P., & Marambaud, P. (2008) · BMC Research Notes · doi:10.1186/1756-0500-1-38 · Cited by 31
“hominis and Acholeplasma laidlawii [12,13].”
BackgroundMycoplasma detection by PCR analysis
Hopert, A., Uphoff, C. C., Wirth, M., Hauser, H., & Drexler, H. G. (1993) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/bf02634350 · Cited by 29
Performance of PCR-based and Bioluminescent assays for mycoplasma detection
Falagan-Lotsch, P., Lopes, T. S., Ferreira, N., Balthazar, N., Monteiro, A. M., Borojevic, R., & Granjeiro, J. M. (2015) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2015.08.010 · Cited by 27
Permanent mycoplasma removal from tissue culture cells: A genetic approach
Mohr, G., Preininger, A., Himmelspach, M., Plaimauer, B., Arbesser, C., York, H., Dorner, F., & Schlokat, U. (2000) · Biotechnology and Bioprocess Engineering · doi:10.1007/bf02931877 · Cited by 3
Mycoplasma Contamination of Primary Cell Cultures
A. Leonovich, O. (2026) · Biochemistry · doi:10.5772/intechopen.1013412 · Cited by 1
Optimized Wound Healing Assay to Study Extracellular Vesicle-Driven Glioblastoma Cell Migration
D’Antonio, C., Mantile, F., Pocsfalvi, G., & Liguori, G. L. (2026) · Methods and Protocols · doi:10.3390/mps9030081
“As Mycoplasma is an invisible contaminant that could alter the structure, metabolism and behavior of the cells [54], thereby affecting the reproducibility of results, we verified its absence in the cell cultures using the Mycoplasma PCR detection kit (Applied Biological Materials Inc., Richmond,…”
Metadata
- Authors
- Hay, R. J., Macy, M. L., & Chen, T. R.
- Venue
- Nature
- Published
- 1989-06
- Volume
- 339
- Issue
- 6224
- Pages
- 487-488
- ISSN
- 0028-0836
- 1476-4687
- Cited by
- 111
- References
- 0
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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