Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein
Abstract
A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of IL-1 to the T-cell/fibroblast form of the IL-1 receptor. Recombinant IRAP specifically inhibited IL-1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL-1 induced corticosterone production in vivo.
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The Il1rn gene encodes four isoforms of IL-1R antagonist (IL-1Ra), which regulates inflammatory signaling of the IL-1 pathway by competing with IL-1α and IL-1β in IL-1R activation.
“The Il1rn gene encodes four isoforms of IL-1R antagonist (IL-1Ra), which regulates the inflammatory signaling of the IL-1 pathway by competing with IL-1α and IL-1β in IL-1R activation”
IL-1Ra binds with IL-1R.
“IL-1Ra binds with IL-1R [27, 30]”
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- Authors
- Carter, D. B., Deibel, M. R., Dunn, C. J., Tomich, C.-S. C., Laborde, A. L., Slightom, J. L., Berger, A. E., Bienkowski, M. J., Sun, F. F., McEwan, R. N., Harris, P. K. W., Yem, A. W., Waszak, G. A., Chosay, J. G., Sieu, L. C., Hardee, M. M., Zurcher-Neely, H. A., Reardon, I. M., Heinrikson, R. L., Truesdell, S. E., Shelly, J. A., Eessalu, T. E., Taylor, B. M., & Tracey, D. E.
- Venue
- Nature
- Published
- 1990-04-12
- Volume
- 344
- Issue
- 6267
- Pages
- 633-638
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2074393911
- Cited by
- 601
- References
- 2
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 8 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 5 · 2018: 5 · 2017: 10 · 2016: 6 · 2015: 7 · 2014: 10 · 2013: 8 · 2012: 8
- FWCI
- 34.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- interleukin-1 receptor antagonist, interleukin-1, IL-1 receptor, cDNA cloning, recombinant protein expression, U937 cells, corticosterone production, T cells, endothelial cells
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Binding, Competitive, Cell Adhesion, Cell Line, Cloning, Molecular, Colony-Stimulating Factors, Corticosterone, DNA, Granulocytes, Growth Substances, Humans, Interleukin-1, Molecular Sequence Data, Monocytes, Neutrophils, Proteins, Receptors, Immunologic, Recombinant Proteins, Sialoglycoproteins, Tetradecanoylphorbol Acetate, Gene Expression, Granulocyte-Macrophage Colony-Stimulating Factor, Receptors, Interleukin-1, Mice, Interleukin 1 Receptor Antagonist Protein
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